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QiM,etal.(2011).PLoSONE宏转录组聚类分析HumanMicrobiomeHowhumanarewe?SimilaritiesbetweenIndividualsSimilaritiesbetweenIndividualsEachbodysitecontainsadistinctMicrobiomeACatalogofReferenceGenomesfromtheHumanMicrobiomeFig.1.Phylogenetictreeof16SrDNAsequences.Thetreewascreatedusing~150016SrDNAsrepresentingsinglespecies.OrganismssequencedaspartoftheHMPprojectarehighlightedinblue.Additionalcoloringindicatesseparationbyphylum:yellow,Actinobacteria;darkgreen,Bacteroidetes;lightgreen,Cyanobacteria;red,Firmicutes;cyan,Fusobacteria;darkred,Planctomycetes;gray,Proteobacteria;magenta,Spirochaetes;lightpink,TM7;tan,Tenericutes.Thepurposeofthisanalysisisnotthedetailsofthebranchingstructure(whichincludeminorknownartifacts),buttheoveralldistributionoftheHMPstrains(inblue)aroundthetreeoflife./content/sci/328/5981/994.full.pdfMetaHIT127AhumangutmicrobialgenecatalogueestablishedbymetagenomicsequencingVol464|4March2010|doi:10.1038/nature08821128PredictedORFsinthehumangutmicrobiomea,Numberofuniquegenesasafunctionoftheextentofsequencing.ThegeneaccumulationcurvecorrespondstotheSobs(MaoTau)values(numberofobservedgenes),calculatedusingEstimateS21(version8.2.0)onrandomlychosen100samplesb,Coverageofgenesfrom89frequentgutmicrobialspeciesc,Numberoffunctionscapturedbynumberofsamplesinvestigated--known(wellcharacterized)orthologousgroups(OGs;bottom),--knownplusunknownorthologousgroups(including,forexample,putative,predicted,conservedhypotheticalfunctions;middle)--orthologousgroupsplusnovelgenefamilies(.20proteins)recoveredfromthemetagenome(top).Boxesdenotetheinterquartilerange(IQR)betweenthefirstandthirdquartiles(25thand75thpercentiles,respectively)andthelineinsidedenotesthemedian.Whiskersdenotethelowestand
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