阿尔茨海默病患者的脑脊液tau蛋白及Aβ1-42水平.pdfVIP

阿尔茨海默病患者的脑脊液tau蛋白及Aβ1-42水平

单守勤;赵权;张立营;孙英姿

【摘要】目的探讨脑脊液(cerebrospinalfluid,CSF)中tau蛋白及β-淀粉样蛋白

1-42(Aβ1-42)对诊断阿尔茨海默病(Alzheimersdisease,AD)的早期诊断价值,寻

找AD理想的生物学标志.方法采用酶联免疫吸附法检测36例AD、24例血管性

痴呆患者(vasculardementia,VD)和26名正常对照者(对照组)CSF中tau蛋白及

Aβ1-42浓度的变化.结果CSF中tau蛋白平均浓度:AD组(336±126)ng/L,VD组

(183±96)ng/L,对照组(98±54)ng/L,AD组CSF中tau蛋白浓度明显高于对照组,

差异有统计学意义(P0.05);CSF中Aβ1-42平均浓度:AD组(341β113)ng/L,VD

组(457±132)ng/L,对照组(502±167)ng/L,AD组CSF中Aβ1-42浓度明显低于对

照组.差异有显著性意义(P0.05).结论脑脊液Aβ1-42、tau蛋白浓度的变化,不仅

对诊断阿尔茨海默病具有较高的敏感性和特异性,而且亦可作为阿尔茨海默病与血

管性痴呆鉴别诊断的生物学指标.%ObjectiveToinvestigatetherelationship

betweenimpairedhippocampalneurogenesisandalteredNotchlsignaling

indepressedrats.MethodsInthepresentstudy,chronicunpredictable

mildstress(CUMS)wasusedtoinhibittheneurogenesisinthe

hippocampus.ThefunctionofNotchlsignalingwasinvestigatedbyusing

realtimePCRandwesternblotatdifferenttimepoints(14dand28d)

duringchronicstress.Thehippocampalneurogenesiswasmonitoredby

assessingcellproliferation,survival,anddifferentiation.ResultsAfter14

days,CUMSsignificantlyreducedweight(P0.05),thesucrosepreference

(P0.001),numberofsquarescrossed(P0.01)andnumberof

groomingandrearingcomparedwiththecontrols.Theimmobilitytime

wassignificantlyincreasedafter14dCUMStreatedrelativetothecontrols

(P0.001).Twenty-eightdaysafterCUMSprotocol,theseparameters

weresignificantlydifferenceinratsexposedtoCUMScomparewiththe

controls(weight,P0.05;sucrosepreference,P0.001;numberof

squarescrossed,P0.001;numberofgroomingandrearing,P0.001;

andimmobilitytime,P0.001).Duringstress,thecellproliferationand

survivalinDGweredecreasedcomparedwiththecontrol(P0.001).The

mRNAandproteinlevelsofNotchlsignalingcomponents(NICD,Hes1,

Hes5,Jag1)inthehippocampusdecreasedduringstress(P0.001)