氧诱导新生小鼠视网膜病变中血管新生和氧应激相关基因表达的变化.pdfVIP

氧诱导新生小鼠视网膜病变中血管新生和氧应激相关基因表达

的变化

余增洋;龚陈媛;张国庆;季莉莉

【摘要】AimToobservetheretinalangiogenesisanddetectthealtered

expressionofgenesrelatedwithangiogenesisandoxidativestressduring

thedevelop-mentofoxygen-inducedretinopathy(OIR)innewbornmice.

MethodsOIRwasestablishedinnewbornmiceaccordingtotheprotocol

ofSmithetal.Newbornmiceat7daysoldwereplacedinto75.5%

oxygenforupto5days,andthentheywereputinroomairforanother5

days.Retinalneovascularizationwasob-servedbyimmunofluorescence

stainingwithclusterofdifferentiation31(CD31).Geneexpressionwas

de-tectedusingReal-timePCRanalysis.RetinalCD31immunofluorescence

stainingassayshowedthatrelativehypoxiainducedretinal

neovascularizationinOIRmiceafterhyperoxia-inducedsubsideofretinal

microvascu-lar.ResultsReal-timePCRanalysisshowedthatvas-cular

endothelialgrowthfactor(VEGF)anditsrecep-tor(VEGFR)suchasVEGFA,

VEGFD,VEGFR1,VEGFR2geneexpressionwereincreasedinOIR

mousenbsp;ascomparedtocontrol.Platelet-derivedgrowthfactor

(PDGF)anditsreceptor(PDGFR)suchasPDGFA,PDGFB,PDGFRa,

PDGFRbgeneexpressionwasalsoincreasedinOIRmouseascomparedto

control.Matrixmetalloproteinases(MMPs)suchasMMP2geneex-

pressionwereincreasedinOIRmouseascomparedtocontrol.Gene

expressionsofnuclearfactor-relatedfac-tor(Nrf2)anditsdownstream

genessuchasthetwosubunitsofglutamate-cysteineligase(GCL):the

cata-lyticsubunit(GCLC)andregulatorysubunit(GCLM)wereboth

decreasedinOIRmouseascomparedtocon-trol.ConclusionOurresearch

demonstratesthattheexpressionofgenesrelatedwithangiogenesisisin-

creasedinretinasinthedevelopmentofOIRinmice,whereasthe

expressionofNrf2anditsdownstreamgenesisalldecreased.%目的：建立

氧诱导新生小鼠视网膜病变(oxygen-in-ducedretinopathy,OIR)模型,观察视网

膜血管新生情况,检测OIR病变过程中调控血管新