盐酸氨溴索防治早期放射性肺损伤的实验研究.pdfVIP

中国现代药物应用2013年10月第7卷第19期ChinJModDmgAppl，Oct2013，Vo1．7，No．19

·论著·

盐酸氨溴索防治早期放射伤的实验研究

张洁曼张纬建陈秀英许建华

【摘要】目的探讨盐酸氨溴索对小鼠早期放射性肺损伤的防治作用。方法成年雄性昆明种小

鼠36只，随机分成对照组，模型组和盐酸氨溴索组，每组12只。对照组小鼠无照射无给药，模型组灌

胃生理盐水10ml／(kg·d)，盐酸氨溴索组灌胃盐酸氨溴索El服溶液40mg／(kg·d)，连续5d。第5天给药

后1h，模型组和盐酸氨溴索组小鼠全肺单次照射18Gvx射线，照射后继续给药3周。于照射后2周、

6周，进行小鼠肺部螺旋CT扫描及图像分析，获得小鼠全肺的平均灰度值；采用HE染色，制作肺组

织病理切片，在光镜下观察肺组织的变化。结果盐酸氨溴索组小鼠全肺平均灰度值明显低于模型组

(尸0．05)；模型组小鼠在照射后存在明显的病理组织学上的改变，表现为肺充血、水肿、肺间质增厚，

盐酸氨溴索组上述所见明显减轻。结论盐酸氨溴索对放射性肺损伤有一定的防治作用。

【关键词】盐酸氨溴索；放射性肺损伤；图像分析r

Preventiveandtherapeuticeffectsofambroxolhydrochlorideonprophaseofradiation-inducedlung

injuryinmiceZHANGJie-min,ZHANGWei-jian,CHENXiu-ying,eta1．DepartmentofPharmacy，TheFist

AffiliatedHospitalofFujianMedicalUniversity,Fuzhou350005,China

【Abstract】objecfiveToinvestigatethepreventiveandtherapeuticeffectsofambroxolhydrochloride

intheearlytimeofradiation—inducedlungjnjuryinmice．MethodsThirtysixKunmingmicewerernadomly

classifiedinto3groups：acontrolgroup．atreatmentrgoupnadaambroxolhydrochloridergoup．Controlgroup

receivedneitherradiationnorambroxolhydrochloride；Treatmentgroupwasorallyadminisrtatednaturalsaline10

ml／(kg．d)nadambroxolhydrochloridergoupwasorMlyadminisrtatedambroxolhydrochloride40mg／(kg‘d)for

5days．Thewholethoraxofrteatmentgroupandambroxolhydrochloridegroupmicewereirradiatedwiht18Gy

X-Rayonehourafteradministerintheffithday，intragastricadminisrtationwerekeptofr3weeksafterirradiation．

CTscanningnadimagenaalysisofthemenagrayvaluewerecarriedouttoacquirethedataofeachmouseafter

irradiationfor2and6weeks．ThepahtologicchnagesoflungwerestudiedbythewayofHEstainingpahtologicof

lung．ResultsComparedwithrteatmentrgoup，htemeanrgayvalueofambroxolhydrochloridegroupobviously

decreased0．05)；significnathistologicalnadpahtologicchangeswereobservedinrteatmentgroup，whileabove