肝癌组织中 HuR 和 HIF-1α的表达及其相关性 .pdfVIP

肝癌组织中HuR和HIF-1α的表达及其相关性

刘利平;江建新;鲍世韵;张育森;何婉

【摘要】目的：观察人抗原R（HuR）和缺氧诱导因子（HIF）-1α在肝癌组织

中的表达变化，并探讨其相关性。方法取48例肝癌组织及癌旁组织标本，采用免

疫组化染色法检测肝癌组织及癌旁组织中HuR和HIF-1α蛋白的表达，采用荧光

定量PCR法检测肝癌组织中HIF-1αmRNA的表达。结果肝癌组织中HuR和

HIF-1α蛋白阳性表达率分别为67％和58％，显著高于癌旁组织的19％和10％

（P均＜0．01）。肝癌组织中HuR与HIF-1α蛋白的表达呈正相关（r＝0．723，

P＜0．01）。HuR蛋白高表达者中HIF-1αmRNA的表达较HuR蛋白低表达者

显著上调（P＜0．05）。结论肝癌组织中HuR和HIF-1α蛋白表达上调且呈正相

关关系，HuR可能增强HIF-1αmRNA的稳定性，从而促进其表达。%Objective

ToinvestigatetheexpressionandcorrelationofhumanantigenR(HuR)

andhypoxia-induc-iblefactor(HIF)-1αinhepatocellularcarcinoma(HCC)

tissues.MethodsForty-eightcasesofHCCtissueandadjacenttissue

sampleswerecollectedfrompatientsundergoingsurgicalresection.The

expressionofHuRandHIF-1αproteinintheHCCandadjacenttissueswas

detectedbyimmunohistochemicalstaining.ThemRNAexpressionofHIF-

1αintheHCCtis-sueswasdetectedbyfluorescentquantitative

PCR.ResultsThepositiveexpressionratesofHuRandHIF-1αproteininthe

HCCtissueswere67%and58%,respectively.Theseratesweresignificantly

higherthanthepositiveexpressionratesofHuRandHIF-1αprotein

(19%and10%)intheadjacenttissues(allP0.01).TheexpressionofHuR

proteinwaspositivelycorrelatedwiththeexpressionofHIF-1αproteinin

theHCCtissues(r=0.723,P0.01).ThemRNAexpres-sionofHIF-1αmRNA

inthegroupwithhighexpressionofHuRproteinwassignificantlyup-

regulatedascomparedwiththatofthegroupwithlowexpressionofHuR

protein(P0.05).ConclusionsTheexpressionofHuRandHIF-1αprotein

wasup-regulatedandpositivelycorrelatedwitheachotherintheHCC

tissues.HuRmayenhancethestabilityofHIF-1αmRNA,andthusincrease

theexpressionofHIF-1αprotein.

【期刊名称】《山东医药》

【年(卷),期】2015(000)018

【总页数】3页(P7-9)

【关键词】肝癌;人抗原R;缺氧诱导因子-1α;RNA结合蛋白

【作者】刘利平;江建新;鲍世韵;张育森;何婉

【作者单位】暨南大学第二临床医学院，深圳市人民医院，广东