乙酸与丙酸比对体外瘤胃液挥发性脂肪酸发酵模式和微生物群体多样性的影.pdfVIP

乙酸与丙酸比对体外瘤胃液挥发性脂肪酸发酵模式和微生物群

体多样性的影响

尹召华;王梦芝;王洪荣;张洁;喻礼怀

【摘要】本文主要研究乙酸与丙酸比(乙/丙)对体外瘤胃液挥发性脂肪酸发酵模式

及微生物群体多样性的影响.试验以4头瘤胃瘘管山羊提供瘤胃液,设4个处理,培养

底物乙/丙分别为30:70、50:50、70:30和100:0.测定培养液中挥发性脂肪酸的浓

度的变化,并通过单链构象多态性(SSCP)图谱检测细菌和原虫的群体多样性.结果表

明:培养液乙酸浓度以100:0组最高,显著高于其他3组(P＜0.05),50:50组最低；

丙酸浓度以30:70组最高,显著高于50:50和70:30组(P＜0.05)；丁酸的浓度

30:70组显著高于70:30组(P＜0.05)；各组培养液乙/丙均接近3:1,30:70组显著

低于70:30和100:0组(P＜0.05).细菌SSCP图谱显示,50:50组条带数最多,100:0

组条带数最少；原虫的SSCP图谱以100:0组条带数最多,30:70组条带数最少.细

菌、原虫的SSCP图谱皆以50:50和70:30组图谱间相似性指数为高.总之,乙/丙

对培养液挥发性脂肪酸模式、细菌或原虫的群体多样性都存在影响.%Thispaper

wasconductedtoinvestigatetheeffectsofacetatetopropionateratioon

thepatternofvolatilefattyacid(VFA)fermentationanddiversitiesof

rumenmicroorganisms.Fourgoatsfittedwithpermanentruminalcannulas

wereusedtoproviderumenfluidfortheinvitrostudy.Thefour

treatmentsweresetaccordingtotheacetatetopropionateratioinculture

substrates,whichwere30:70,50:0,70:30,and100:0,respectively.Variation

ofVFAconcentrationwasrecorded,anddiversitiesofbacteriaand

protozoawereinvestigatedbySSCPfingerprinttechnique.Theresults

showedasfollows:acetateconcentrationof100:0groupwasthehighest

andwassignificantlyhigherthanthatoftheotherthreegroups(P0.05),

whilethatof50:50groupwasthelowest.Propionateconcentrationof

30:70groupwasthehighestandwassignificantlyhigherthanthatof

groups50:50and70:30(P0.05).Butyrateconcentrationof30:70group

wassignificantlyhigherthanthatof70:30group(P0.05).Acetateto

propionateratiosinculturemediumofallthetreatmentswerecloseto3:1,

whilethatof30:70groupwassignificantlylowerthanthatofgroups70:30

and100:0(P0.05).BacterialSSCPfingerprintshowedthatbandsnumber

of50:50groupwasthemost,whilethatof100:0groupwastheleast;

protozoaSSCPfingerprintshowedthatbandsnumbero