免疫诊断-Immunodiagnosis-精品英文课件.pptVIP

Immunodiagnosis

DetectionofAntigenandantibodies*ThespecificbindingofAg-AbprovidedusagreattoolformodernbiomedicalResearch2.DetectionofCellularImmunity:ActivationofTlymphocytesProliferationcytotoxicitycytokineproduction….Immunodiagnosis

Antigen-AntibodyInteractionAbsareproteinssecretedbyB-lymphocytes.Recognizeandbindtomolecules(antigens)onforeignparticles,markingthemfordestruction.CanbeusedtodetectantigenwithspecificAb;Reciprocally,tousepurifiedantigentodetectspecificAb.SpecificityAffinityDetectionofAntigenorantibodies

IHCWesternBlotfluorescenceFACSmicroscope??CD4CD8Antibodyconjugation-visualizationofAbhorseradishperoxidase

SecondAntibodyAsecondaryantibodyisanantibodythatbindstoprimaryantibodiesorantibodyfragments.Theyaretypicallylabeledwithprobesthatmakethemusefulfordetection,purificationorcellsortingapplications.Specificsecondaryantibodiesareselectedaccordingtothesourceoftheprimaryantibody,theclassoftheprimaryantibody(e.g.,IgGorIgM),andthekindoflabelwhichispreferred.Oneconjugated2ndantibodycandetectallprimaryantibodieswithsameisotype,whichreducethelabortoconjugateallprimaryantibodies

SecondAntibody

Agglutination(aggregation)Assays:ImmunodiffusionComplementFixationEIA(EnzymeImmunoassay)(IHC/ELISA/ELISPOT)Immunofluorescence(IFAFACS)ICS（Intracellularcytokinestaining）RadioimmunoassayCLIA（Chemiluminescenceimmumoassay）TraditionalImmunoassaysModernImmunoassays(immunolabelling)Immunoassays

ImmunoassaysEIA(EnzymeImmunoassay)SubstrateColorDetection

IHC

ELISA

TheEnzyme-linkedimmuno-sorbentspot(ELISPOT)assayisacommonmethodformonitoringimmuneresponsesinhumansandanimals.ItwasdevelopedbyCecilCzerkinskyin1983.TheELISPOTassayisbas