总磷的测定-Determination of Total Phosphorou80课件讲解.pptx

总磷的测定DeterminationofTotalPhosphorous主讲教师：杨文婷Lecturer:YangWenting设备与环境学院建筑环境工程教学团队TeachingTeamofBuildingEnvironmentalEngineering,SchoolofEquipmentandEnvironment

课程导入Introduction总磷是水中的各种形态的磷转变成正磷酸盐的总数，总磷是水体富含有机质的指标之一。磷含量过多会引起藻类植物的过度生长，水体富营养化，打乱水体的平衡，因此检测水中的含磷量十分重要。Totalphosphorusrepresentsthesumofvariousformsofphosphorusinwaterthathavebeenconvertedintoorthophosphate,anditisoneoftheindicatorsthatawaterbodyisrichinorganicmatter.Excessivephosphoruscontentcanleadtotheovergrowthofalgae,waterbodyeutrophicationanddisruptionofwaterbalance.Therefore,itisveryimportanttodetectthephosphoruscontentinwater.

实验目的ExperimentalPurposes01

一、实验目的ExperimentalPurpose01了解磷（总磷、溶解性正磷酸盐和溶解性总磷）的测定方法Understandthemethodfordeterminationofphosphorus(totalphosphorus,dissolvedorthophosphateanddissolvedtotalphosphorus)02熟悉钼酸铵分光光度法测定总磷的原理和过程Befamiliarwiththeprincipleandprocessofammoniummolybdatespectrophotometryfordeterminationoftotalphosphorus03掌握分光光度计的使用、标准曲线的绘制及计算方法Mastertheuseofspectrophotometer,drawingofstandardcurveandcalculationmethod

实验原理ExperimentalPrinciples02

在酸性介质中，正磷酸盐与钼酸铵反应，在锑盐存在下生成磷钼杂多酸后，立即被抗坏血酸还原，生成蓝色的络合物。该络合物在700nm处有最大吸收，据此可分析水中的总磷。Inacidicmedium,orthophosphatereactswithammoniummolybdatetoformaphosphomolybdicheteropolyacidinthepresenceofantimonysalt.Itisthenpromptlyreducedbyascorbicacidtoproduceabluecomplex.Thecomplexexhibitsmaximumabsorptionat700nm,accordingtowhichtotalphosphorusinwatercanbeanalyzed.PO43-+钼酸盐+抗坏血酸PO43-+molybdate+ascorbicacidH+蓝色络合物（磷钼蓝）Bluecomplex(phosphorusmolybdenumblue)二、实验原理ExperimentalPrinciples

实验仪器及试剂ExperimentalInstrumentsandReagents03

三、实验仪器及试剂Experimentalinstrumentsandreagents分光光度计Spectrophotometer容量瓶Volumetricflask比色管Colorimetrictube比色皿Cuvette移液管Pipette烧杯若干Beakers实验仪器ExperimentalInstruments*注：所有玻璃器皿均应用稀盐酸或稀硝酸浸泡*Note:Allglasswareshallbesoaked