硫通过促进小白菜同化谷胱甘肽代谢减少镉提高耐受性修改后.pdfVIP

Sulfurdecreasescadmiumtranslocationandenhancescadmium

tolerancebypromotingsulfurassimilationandglutathione

metabolisminBrassicachinensisL.

TaishuaiLiangandJinyinLv*

CollegeofLifeSciences,NorthwestAFUniversity,Yangling,Shaanxi712100,PR

CollegeofScience,NorthwestAFUniversity,Yangling,Shaanxi712100,PR

Weinvestigatedtheameliorativeroleofsulfur(S)inprotectingplantsagainstcadmium(Cd)

toxicitybyusinopakchoi(BrassicachinensisL.)cultivarswithdifferentCdtolerancelevels.The

exposureofpakchoiseedlingsto100μMCdinhibitedplantgrowth,increasedsuperoxidecontent,

enhancedmembranelipidperoxidation,andinducedCdaccumulationintherootsandshoots.

ApplicationofStoCd-stressedplantsalleviatedCd-inducedoxidativestressbypromotingthe

capacityoftheascorbate(AsA)–glutathione(GSH)cycle,enhancedSassimilationbyincreasing

theactivityofATPsulfurylaseando-acetylserine(thiol)lyase,anddecreasedCdtranslocationfrom

therootstotheshootsbyenhancingphytochelatin(PC)biosynthesis.ResultssuggestedthatS

reversedCd-inducedgrowthinhibitionandoxidativestressbyrestrainintranslocationfromthe

rootstotheshootsandupregulatingSassimilationandGSHmetabolismthroughtheAsA–GSH

cycleandPCsynthesis.

Keywords:Sulfur;Cadmium;Pakchoi;Cdtranslocation;Sulfurassimilation;GSHmetabolism

1.Introduction

Withrapidindustrialandagriculturaldevelopment,largeareasofcultivatedsoilsworldwide

havebeencontaminatedbycadmium(Cd)becauseofexcessiveutilizationofchemicalfertilizers,

wastewaterirrigation,andsewagesludgeinthepastdecades(Zhangetal.,2013a;Fanetal.,2010;

Liangetal.,2014).Cd,oneofthemostw