-茯砖茶中真菌的分离鉴定.docx

PAGE

PAGE1

茯砖茶中真菌的分离鉴定

摘要：【目的】探究茯砖茶中优势真菌的种类及形态学特征，为茯砖茶发花过程中实现人工干预促进优势真菌生长，提高茯砖茶品质，实现标准化生产提供理论依据。【方法】本实验以茯砖茶“发花”过程样（第9d）作为实验材料，通过真菌分离纯化技术对茯砖茶中的真菌进行分离纯化，平板培养进行菌落形态学观察、ITS区域序列同源性比对与系统发育分析方法对其进行聚类鉴定。【结果】结合形态学鉴定和分子生物学鉴定结果，将分离纯化得到的6种真菌鉴定为：金花菌（Aspergilluscristatus）、琉球曲霉（Aspergillusluchuensis）、米曲霉（Aspergillusoryzae）、皮落青霉（Penicilliumcrustosum）、灰绿曲霉（Aspergullusglaucus）、黑曲霉（Aspergillusniger）【结论】茯砖茶“发花”过程样（第9d）中优势真菌主要集中于曲霉属、青霉属真菌，以上种属的特定作用，促进了茯砖茶独特风味品质的形成。

关键词：发花；优势真菌；形态学特征；ITS序列分析

IsolationandidentificationoffungiinFuBricktea

Abstract:【Objective】ToexplorethespeciesandmorphologicalcharacteristicsofthedominantfungiinFuBrickteatea,soastoprovideatheoreticalbasisforartificialinterventiontopromotethegrowthofthedominantfungi,improvethequalityofFuBrickteateaandachievestandardizedproduction.【Method】Inthisexperiment,thesampleofthe“blooming”processofFuBricktea(No.9D)wastakenastheexperimentalmaterial.ThefungiinFuBrickteawereseparatedandpurifiedbythetechniqueoffungiseparationandpurification.Thefungiweregroupedandidentifiedbythemethodsofcolonymorphologyobservation,ITSregionalsequencehomologycomparisonandphylogeneticanalysisinplateculture.【Results】Combinedwiththeresultsofmorphologicalidentificationandmolecularbiologicalidentification,thesixfungiisolatedandpurifiedwereidentifiedas:Aspergilluscristatus、Aspergillusluchuensis、Aspergillusoryzae、Penicilliumcrustosum、Aspergullusglaucus、Aspergillusniger.【Conclusion】Thedominantfungiinthe“blooming”processofFuBricktea(No.9D)weremainlyAspergillusandPenicilliumfungi.ThespecificeffectsofthesefungipromotedtheformationoftheuniqueflavorqualityofFuBricktea.

Keywords:Hairflower;Dominantfungi;Morphologicalcharacteristics;ITSsequenceanalysis

目录

TOC\o1-3\h\u911引言 1

168222材料与方法 2

112322.1实验材料 2

91192.2实验主要试剂 2

58522.3实验主要仪器与设备 2

302542.4实验方法 2

50642