案例reserpine专着4535 tablets4534-治疗片.pdfVIP

4534Reserpine/OfficialMonographsUSP35

Assay—Alcoholcontent〈611〉:between11.0%and13.0%of

CHOH.

Standardpreparation—Dissolve25.0mgofUSPReserpineRS,25

accuratelyweighed,in0.25mLofchloroform,mixwithaboutAssay—

30mLofmethanolpreviouslywarmedto50°,transferthemix-Standardpreparation—Dissolve25.0mgofUSPReserpineRS,

turetoa250-mLvolumetricflaskwiththeaidofwarmmetha-accuratelyweighed,in0.25mLofchloroform,mixwithabout

nol,coolthesolutiontoroomtemperature,dilutewithmetha-30mLofmethanolpreviouslywarmedto50°,transferthemix-

noltovolume,andmix(Solution1).Protectthesolutionfromturetoa250-mLvolumetricflaskwiththeaidofwarmmetha-

light.JustpriortouseintheAssay,pipet10mLofSolution1nol,coolthesolutiontoroomtemperature,dilutewithmetha-

intoa50-mLvolumetricflask,add36mLofchloroform,andnoltovolume,andmix(Solution1).Protectthesolutionfrom

dilutewithmethanoltovolume(Standardpreparation).light.JustpriortouseintheAssay,pipet10mLofSolution1

Assaypreparation—Transfertoa100-mLvolumetricflaskanintoa50-mLvolumetricflask,add36mLofchloroform,and

accuratelymeasuredvolumeofInjection,equivalenttoaboutdilutewithmethanoltovolume(Standardpreparation).

10mgofreserpine,anddilutewithcitric