一氧化氮合酶抑制剂对延缓腰椎间盘退变的影响.pdf

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一氧化氮合酶抑制剂对延缓腰椎间盘

退变的影响

李小川付勤贾长青王海义

[摘要】目的探讨一氧化氮合酶(NOS)抑制剂L-N6一亚氨乙基一赖氨酸(L-NIL)和S-甲基异

硫脉(SMT)对退变腰椎间盘组织代谢的影响。方法无菌条件下，取20例腰椎间盘突出症患者的

椎间盘组织体外培养，分别加入1mmol/L浓度的SMT和L-NIL，培养72h后，通过检测硝酸盐和

亚硝酸盐的含量来观察椎间盘NO的释放量及NOS的活性;原位杂交法检测椎间盘组织iNOS

mRNA和MMP3mRNA的表达。培养10d后，化学比色法观察椎间盘蛋白多糖含量和羚脯氨酸

释放量的变化。结果L-NIL组髓核和纤维环NO释放2(65.614.5,68.815.7)umol/L和SMT

组髓核和纤维环NO释放量(69.5士6.5,69.1士6.1)tcmol/L较对照组NO释放量(107.9士4.4,

93.1士5.9)pmol/L明显减少(P0.01)aL-NIL组和SMT组髓核组织中蛋白多糖含量((51.3士

9.6,48.2士8.5)kg/L，比对照组((32.1士6.4)kg/L明显增加(尸0.01),9脯氨酸释放量(1.1士

0.4,1.2士0.5)kg/L比对照组(3.4士0.8)kg/L显著减少(尸0.01);同时，原位杂交法未检测到

iNOSmRNA和MMP3mRNA的表达。结论NOS抑制剂L-NIL和SMT能抑制过量NO的释

放，对延缓椎间盘退变具有积极的作用。

【关键词】椎间盘;退行性变;一氧化氮合酶

Potentialclinicalevaluationofnitricoxidesynthaseinhibitorforthetreatmentoflumbarintervertebral

discdegenerationLIXiao-chuan,FUQin，JIAChang-ging,etal.DepartmentofOrthopaedic

Surgery,theSecondAffiliatedHospitalofChinaMedicalUniversity,Shenyang110004,China

[AbstractlObjectiveTodiscussthemetaboliceffectsondegenerativelumbarintervertebraldisc

byNOSinhibitorL-N6-(sub-amonion-ethyl)lysine(L-NIL)andSmethylisothiourea(SMT).Methods

Twentydegenerativelumbarintervertebraldiscswereobtainedfrompatientsundergoingdiscsurgery.

Thespecimenswereculturedanddividedintothreegroups.L-NILandSMTgroupwereinterferedwith

NOSinhibitorL-NILandSMTrespectively.After72hculture,thereleaseofNOandtheactivityof

NOSinculturemediaweremeasuredbyGriessreactionandspectrophotometricmethods.iNOSmRNA

andMMP3mRNAexpressionwasdetectedbyinsituhybridization.Proteoglycanandhydroxyprolinein

thesespecimenswasmeasuredwithcolorimetricanalysisafter10dayculture.ResultsTheNOconcen-

trationofnucleuspulposus(NP)andanulusfibrosus(AF)inL-NILgroups.[(65.614.5,68.815.7)

ymol/Lrespectively]andinSMTgroups(〔69.516.569.116.1)pm胡Lrespectively]weresignifi-

cantlyles