HFD对不同性别SD大鼠肠道微生物及室旁核小胶质细胞激活的影响.pdf

目录

中文摘要·······································································4

英文摘要·······································································6

英文缩写·······································································9

研究论文

HFDSD

对不同性别大鼠肠道微生物及室旁核小胶质细胞激活的影

响

前言·······································································10

材料与方法·······························································12

结果·······································································24

附图·······································································28

附表·······································································33

讨论·······································································36

结论·······································································42

参考文献··································································43

综述··········································································50

致谢··········································································76

个人简历·······································································77

3

HFD对不同性别SD大鼠肠道菌群及室旁核小胶质细胞激活的

影响

摘要

本研究通过建立雌、雄性SD（Spraguedawley,SD）大鼠模型，

研究高脂饮食（Highfatdiet,HFD）对不同性别大鼠肠道微生物及室旁

核（Hypothalamicparaventricularnucleus,PVN）区小胶质细胞激活的影

响。

选取24只3周龄的SD大鼠，雌雄各半，随机分成雄性对照组（CM）、

雌性对照组（CF），雄性高脂组（HM）、雌性高脂组（HF），共4

组，每组6只。喂养至10周龄，收集大鼠新鲜粪便并提取细菌总基因

组DNA，通过PCR扩增16S-rDNA的V3-V4区域，进行高通量测序；

采用气相色谱法分析大鼠盲肠内容物中短链脂肪酸（Shortchainfatty

acids,SCFAs）含量；以Real-timePCR技术分析PVN区小胶质细胞

激活标记物CD11b和炎性细胞因子TNF-α、IL-6的mRNA相对表达水

平。

雌、雄高脂组分别与对照组相比，大鼠肠道微生物总数及菌群多

样性均明显减少（P0.05）；而HF组较CF组的菌群丰富度明显减少

（P0.05）。与CM组相比，HM组大鼠肠道微生物总数及菌群多样性

明显减少（P0.05）。在门水平上，HM组