G25脱盐柱使用指南.pptVIP

G25脱盐柱使用指南

MatrixSephadex?G-25Fine,cross-linkeddextranBedvolume53mlBedheight100mmi.d.26mmseparationofhigh(Mr5000)fromlowmolecularweightsubstances(Mr1000).Storage+4to+30°20%ethanol2

长期不用后再使用，应该用5CV平衡柱子（5*60=300mlbuffer）TrytheseconditionsfirstBuffer(35ms/cm):50mMphosphatebuffer,0.15MNaCl,pH7.0Flowrate:20ml/minatroomtemperatureSamplevolume:15mlSeparations:Elutethecolumnwith106ml(2CV)ofbuffer3

Equilibrationisnotnecessarybetweenrunswiththesamebuffer.(去除气泡）Reversetheflowdirectionandpump200mlwellde-gassedbufferataflowrateof15ml/minthroughthecolumn.Thequalityofthepackedbedwillnotnormallybeaffected.4

5

6

7

大家应该也有点累了，稍作休息大家有疑问的，可以询问和交流8

SephadexG-10iswellsuitedfortheseparationofbiomoleculessuchaspeptides(Mr700)fromsmallermolecules(Mr100).SephadexG-50issuitablefortheseparationofmoleculesMr30000frommoleculesMr1500suchaslabeledproteinorDNAfromunconjugateddyes.Themediumisoftenusedtoremovesmallnucleotidesfromlongerchainnucleicacids.SephadexG-25isrecommendedforthemajorityofgroupseparationsinvolvingglobularproteins.ThesemediaareexcellentforremovingsaltandothersmallcontaminantsawayfrommoleculesthataregreaterthanMr5000.9

samplevolumesofupto30%ofthetotalvolumeofthedesaltingcolumncanbeprocessed.notexceedapproximately70mg/ml。10

谢谢大家！ThankYou！