免疫学概论及分子生物学在免疫学中的应用.pptVIP

FangLi,GuizhiCheng,HongQu,BingWang,JieMa,Shiyingwang

Dept.ofImmunology

Collegeofbasicmedicalsciences

DalianMedicalUniversity

Tel:

;I.IntroductionToImmunology

II.ApplicationofMolecularBiologyinImmunology;CHAPTERI;Immunology;TheImmuneSystemisthenameofacollectionofMolecules,CellsandOrganswhosecomplexinteractionsformanefficientsystemthatisusuallyabletoprotectanindividualfrombothoutsideinvadersanditsownalteredinternalcells.;;中枢免疫器官（Centrallymphoidorgan）;外周免疫器官（Peripherallymphoidorgan）;;第十页，共一百一十页。; 单核巨噬细胞;第十二页，共一百一十页。;;抗体（antibody）;;;;;;;免疫记忆——疫苗与抗感染免疫;Immunologicalmemorymakesvaccinationagainstinfectiousdiseasespossible!;;;免疫记忆Immunologicmemory;第二十六页，共一百一十页。;;;临床免疫及免疫病理——;IHypersensitivity超敏反应;第三十一页，共一百一十页。;Contactallergylimitedtotheeyelids,duetosecondarycontactfromhairdyecontainingparaphenylenediamine;;Eczema

湿疹;Serumsickness;;5;6.;T、B细胞联合免疫缺陷

SevereCombinedImmunodeficiency，SCID;第四十页，共一百一十页。;HIV感染淋巴细胞;HIVbuddingfromhumanlymphtissue(TEMx133,335);LesionsonthestomachofapatientwithKaposissarcoma?;Hairyleukoplakia（黏膜白斑）oftongueinAIDS;RA患者手指关节实质性损伤;;ankylosingspondylitis(B27),;Destructionofpancreaticislet?-cellsbyinfiltratingT-cellsintheNonobesediabetic(NOD)mouse;ApplicationofMolecularBiologyinImmunology;MOLECULARBIOLOY;Practicalapplications

inthedevelopmentofnewandsafeproductssuchastherapeutics,vaccinesandfoodstuffs,andinthediagnosisofgeneticdiseaseandingenetherapy;一、新细胞因子的cDNA克隆技术;;;;;第二轮杂交，加新的变性driver,退火;第五十八页，共一百一十页。;;第六十页，共一百一十页。;第六十一页，共一百一十页。;第六十二页，共一百一十页。;第六十三页，共一百一十页。;第六十四页，共一百一十页。;GTTCCCAATCTGAAGTGAAGCCGAGCTGGGCGAGAAGTAGGGGAGGGCGGTGCTCCGCCG60

CGGTGGCGGTTGCTATCGCTTCGCAGAACCTACTCAGGCAGCCAGCTGAGAAGAGTTGAG120

GGAAAGTGCTGCTGCTGGGTCTGCAGACGCGATGGATAACGTGCAGCCGAAAATAAAACA180

MDNVQPKIKH

TCGC