CBX6通过抑制SNAI2表达降低胶质瘤细胞侵袭.pdfVIP

年第卷第期Vol.No.Jun1801

医学理论与实践2025381138,11,2025JMedTheorPrac

CBX6通过抑制SNAI2表达降低胶质瘤细胞侵袭∗

邓后亮莫小兰

广州医科大学附属妇女儿童医疗中心药学部,广东省广州市510120

摘要目的:研究CBX6对胶质瘤细胞侵袭的影响及分子机制。方法:构建稳定过表达CBX6胶质瘤细胞。transwell

实验检测胶质瘤细胞侵袭性。qRT-PCR和蛋白质印迹法分别检测基因mRNA和蛋白表达水平。免疫组化检测人胶

质瘤组织蛋白表达水平。Pearson相关分析胶质瘤组织基因表达量相关性。在过表达下游靶基因的情况下,transwell

实验检测CBX6对胶质瘤细胞侵袭性的影响。结果:过表达CBX6显著降低胶质瘤U251MG细胞侵袭能力。机制研

究发现CBX6抑制SNAI2表达。在胶质瘤组织中CBX6表达量和SNAI2表达量呈负相关,而且过表达SANI2可部分

逆转CBX6对U251MG细胞侵袭的抑制作用。结论:CBX6可能通过沉默SNAI2表达抑制胶质瘤细胞侵袭。

关键词胶质瘤细胞侵袭CBX6SNAI2上皮—间质转化

中图分类号:R73-3文献标识码:Adoi:10.19381/j.issn.1001-7585.2025.11.001

CBX6ReducesInvasionofGliomaCellThoughRepressingSNAI2Expression

DENGHouliang,MOXiaolan.DepartmentofPharmacy,GuangzhouWomenandChildren’sMedicalCenter,Guangzhou

MedicalUniversity,GuangzhouCity,GuangdongProvince510120

ABSTRACTObjective:ToinvestigatetheeffectsofCBX6ontheinvasionofgliomacellandexplorethepotentialmolecular

mechanisms.Methods:ThestableoverexpressionofCBX6ingliomacellwasconstructed.Gliomacellinvasionwasdetectedby

transwellassay.mRNAandproteinexpressionlevelsweredetectedbyqRT-PCRandWesternblot,respectively.Immunohisto-

chemistrywasusedtodetecttheproteinexpressionofhumangliomatissues.Pearsoncorrelationwasusedtoanalyzethecorre-

lationofgeneexpressionlevelinglioma.Inthecaseofoverexpressionofdownstreamtargetgene,theeffectofCBX6onthein-

vasionofgliomacellwasdetectedbytranswellassay.Results:OverexpressionofCBX6significantlydecreasedtheinvasiona-

bilityofU251MGcell.FunctionalassaysdemonstratedthatCBX6couldrepressSNAI2expression.Therewasasignificantly