响应面法优化超临界CO_(2)萃取白扁豆挥发油工艺及其成分分析.pdfVIP

36粮食与油脂2025年第38卷第8期

响应面法优化超临界CO，萃取

白扁豆挥发油工艺及其成分分析

罗依2-3，杨芳芳，徐剑2-3，张永萍123，刘杰23

（1.贵州中医药大学药学院，贵州贵阳550025；

2.国家苗药工程技术研究中心，贵州贵阳550025；

3.贵州中药炮制与制剂工程技术研究中心，贵州贵阳550025)

摘要：采用超临界CO,萃取白扁豆中挥发油。以挥发油得率为指标，在单因素试验的基础上，

通过响应面试验优化萃取工艺，并运用气相色谱-质谱联用（GC-MS）分析挥发油成分。结果表明：

最优萃取工艺为萃取时间3.0h、萃取压力25MPa、萃取温度50℃，在此条件下白扁豆挥发油得率为

1.37%

表明白扁豆挥发油具有多种潜在的生物活性，可用于开发优质药材资源及保健品。

关键词：白扁豆挥发油；超临界CO2提取；工艺优化；响应面法

OptimizationofsupercriticalCO2extractionofhyacinthbeanvolatileoil

byresponsesurfacemethodologyandanalysisofitscomponents

LUOYr2.,YANGFangfang,XUJian-3,ZHANGYongping-,LIUJiel.

(1.SchoolofPharmacy,GuizhouUniversityofTraditionalChineseMedicine,

Guiyang550025,Guizhou,China;

2.NationalEngineeringandTechnologyResearchCenterforMiaoMedicine,

Guiyang550025,Guizhou,China;

3.GuizhouEngineeringResearchCenterforChineseMedicineConcoction

andPreparation,Guiyang550025,Guizhou,China)

Abstract:ThevolatileoilfromhyacinthbeanwasextractedbysupercriticalCO2.Takingtheyieldofthe

volatileoilastheindex,theextractionprocesswasoptimizedthroughresponsesurfaceexperimentbased

onsinglefactorexperiments.Andthecomponentsofthevolatileoilwereanalyzedbygaschromatogra-

phy-massspectrometry(GC-MS).Theresultsshowedthattheoptimalextractionprocesswereasfollows:

extractiontime3.0h,extractionpressure25MPa,andextractiontemperature50C.Underthesecondi-

tions,theyieldofhyacinthbeanvolatileoilwas1.37%.Inhyacinthbeanvolatileoil,atotalof43

chemicalcomponentswereidentified.Amongthem,theproport