CypHer-5-solution-生命科学试剂-MCE.pdfVIP

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CypHer5(solution)

Cat.No.:HY-DY1071

CASNo.:312961-83-4

分⼦式:C₃₁H₃₆N₂O₈S₂

分⼦量:628.76

作⽤靶点:FluorescentDye

作⽤通路:Others

储存⽅式:Pleasestoretheproductundertherecommendedconditionsin

theCertificateofAnalysis.

BIOLOGICALACTIVITY

⽣物活性CypHer5(solution)是⼀种对pH敏感的花青衍⽣物和对环境敏感的荧光基团。CypHer5对pH敏感的最⼤吸

收波长为644nm，发射波长为664nm。CypHer5已被⽤作溶酶体腔室的pH传感剂。

溶剂及浓度：DMSO：10mM

体外研究CypHer5technologyutilizesthegeneralabilityofGPCRstobeinternalizedintotheendosomalpathwayofa

cellinresponsetoagonistligands.TheCypHer5dyeisfluorescentinacidicenvironments,but

nonfluorescentatneutralpH.WhenCypHer5isboundtoareceptorontheextracellularsurfaceofthecell,it

isessentiallynonfluorescent.Oninternalizationintoacell,itdisplaysasignificantincreaseinfluorescence

[1].

REFERENCES

[1].PatrickMJ,etal.IntracellularpHmeasurementsusingperfluorocarbonnanoemulsions.JAmChemSoc.201