临床检测 资料Clin-Chem Technology Principle_VN201507.pptxVIP

;Photometricprinciple

Photometriccalibrationdataalarms

ISEmeasurementprinciple

ISEcalibrationdataAlarms;Photometricprinciple

Photometriccalibrationsdataalarms

ISEmeasurementprinciple

ISEcalibrationsdataalarms

;Mostchemicalreactionsaredesignedtoendina“coloured”product

Photometryisthe“Measurementoflight“,Lightabsorbanceofmoleculesaremeasuredwithinthevisibleandultra-violet(UV)spectralrange.;Absorbance(Abs)ismoreconvenienttocalculateconcentrationthantransmittance%.

TheAbsisdirectlyproportionaltoconcentration. ;Photometerandlightpath;TheLambert-Beerlawdescribesthelinearrelationshipbetweenabsorbanceandconcentration;Lambert-Beer’sLaw;Lambert-Beer’sLaw;;光度计测量范围与比色杯光径;双波长检测改善检测精密度;在罗氏仪器上，待测物的12个波长的吸光度都被检测，选择设定中的2个波长进行计算。;ConceptofseveralBlank;Performeddailyasmaintenance

Checksintegrityoflamp

WaterblankabsorbanceofcuvetteNo.1atall12wavelengths

;PhotometerCheck;CellBlankMeasurements;StoredCellBlank

measured/updatedasapartoftheweeklymaintenance

Storedintheinstrument’smemory

Givesinformationabouteachindividualcellforeachwavelength

Compensatesforthecuvettewearwithtime

Acellwashmusttobeperformedbeforecellblankmeasurement

Iftherearecellsoutoflimits,theconcernedcellsarelistedintheAbnormalCellList;CellBlankMeasurements;CellBlankMeasurements;Cellpreparation-rinsingandcellblankmeasurement

Pipettingofsampleintoareactioncell

Pipettingoffirstreagentandmixingofreagentandsampleinthecell

Reaction-Ateachfullcycleofthereactiondisktheabsorbanceismeasuredforeverycell

Pipettingofsecond/thirdreagentandmixinginthecell(test-dependent)

Reaction-Ateachfullcycleofthereactiondisktheabsorbanceismeasuredforeverycell

Themeasuredresultsarecalculatedandsavedafterthespecificreactiontimehaspassed

Afterthemeasurementiscomplete,Thereactionsolutionis