用流式细胞仪分析脂肪细胞.pptVIP

用流式细胞仪分析脂肪细胞

2CONTENTSBackgroundAimoftheresearchDifficultySolution

BackgroundWhatareadipocytes(fatcells)?

Majorcellsintheadiposetissue.3

BackgroundLipiddropletTwokindsofadipocytesinhuman4

Prospectsofadipocytebiology

BackgroundLipidstoreInhibiteatingIncreasemetabolismLeptin5

Prospectsofadipocytebiology

BackgroundAdipokinesInvolvedinenergymetabolismLipidstore6

Prospectsofadipocytebiology

Specificallyfromadipose

tissue

BackgroundLipidstoreAdipokinesEnergymetabolism7

Prospectsofadipocytebiology

Adipokinesplayansignificantroleinbiologicalfunction.

Background8

BackgroundFlowCytometry(FCM)Principlediagram9

BackgroundVolume,Morphologicalcomplexityofcells,TotalDNA/RNAcontent,Apoptosis,Cellviability,............10

AimoftheresearchAmethodforanalysisandisolationfree-floatingadipocytesbyFlowCytometry.AnalysisFlowcytometry(FCM)Confocallaserscanningmicroscope(CLSM)IsolationFluorescence-activatedcellsorting(FACS)11

AimoftheresearchAnalysisFlowcytometry(FCM)Confocallaserscanningmicroscope(CLSM)BeckmanCoulter--MoFlo?XDPAnalysisrate:100,000events/sec.Sortrate:70,000events/sec.12Amethodforanalysisandisolationfree-floatingadipocytesbyFlowCytometry.

AimoftheresearchAnalysisFlowcytometry(FCM)Confocallaserscanningmicroscope(CLSM)QuantitiveHighresolutionimaging13Amethodforanalysisandisolationfree-floatingadipocytesbyFlowCytometry.

AimoftheresearchAnalysisFlowcytometry(FCM)Confocallaserscanningmicroscope(CLSM)ImagingFlowCytometer(Amnis)12ImagesforeverycellQuantitiveHighresolutionimaging14Amethodforanalysisandisolationfree-floatingadipocytesbyFlowCytometry.

AimoftheresearchIsolationFluorescence-activatedcellsorting(FACS)Apassingcellcanbeselectivelyadddifferentcharge,fallingintodifferentcontainers.15Amethodforanalysisandisolationfree-floatingadipocytesbyFlowCytomet