细胞内钙离子浓度测量原理与方法.pptxVIP

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  • 2026-03-25 发布于北京
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ThinkingandTesting(09-15-2008)1、YouareusingacameleonindicatortomeasureintracellularconcentrationsofCa2+.Theindicatoriscomposedofacentralcalmodulindomain,whichconvertsfromanextendedformtoamuchmorecompactformuponcalciumbinding,andtwoflankingfluorescentproteins,withCFPattachedatoneendandYFPattachedattheother.YouhaveexpressedthisindicatorincellsandnowwishtomeasureintracellularchangesinCa2+concentrationinresponsetothebiologicalprocessyouarestudying.Th

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