甘蔗组培苗快速增殖技术研究.PDF

维普资讯 @乡 甘蔗糖业1997年第5期 甘蔗组培苗快速增殖技术研究宰 吴才文蠢廷豪√邹宗兰 陈远贻 s“6、 (四川 究所 ， 四川 641200) Studieson Rapid Propagation ofSugarcane by TissueCulture WuCaiwen QinTinghao Zhou Zhonglan ChenYuanyi (SugarandSugar—yieldingPlantsInd．Res．InstofSichuanProvince．Sichuan641200) 摘 要 甘蔗组培苗经多种培养基进行丛芽增殖 ．筛选出效果好的两种培养基继代 ，设计多种组培苗基数的 增殖试验 ，同时观察温度对组培苗增殖效果的影响。结果表 明：在植物生长调节剂 (本文称 激素”．下同) 含量高低不同的培养基中轮换继代 ．有利于保持组培苗的活力 ；继代基数以3苗以上苗丛增殖数量多；在24 -- 35℃范围内．随着温度升高，丛芽增殖时间提早．速度加快。 关毽词 甘蔗 组培苗 增殖 技术 一 J ～ Abstract The clumpy buds of tissue culture sugarcaneplantletswere pro1iferated in variOUS media and two better media were chosen for subculture． The trialB on appropriate cardinal numbers of plant1ets were earried out The effects of temperature on the increasing rate of tissue culture plant1ets were studied The results shelwed that rotatable subculture in different media vatied in concentrations ofplantgrowth regulators might help maintain vitality of the plantlets The preferablenumber ofplantlets transferred for subculture seemed to be above 3 Proliferation of clumpy budswould start earlierand increasing rate quicken as the culturetemperaturerose in therangeof24—35℃ Keywords Sugarcane；Tissue—culture seedling；Propagation；Technique 本文为省重点项 目 甘蔗优 良品种引进及配套技术研究”的内容之一。 本所甘蔗系主任陈韶武高级工程师对本文进行审阅．在此致谢 !