[分子数量遗传学课件]A mutation creating a potential illegitimate miRNA target site in the myostatin gene affects muscularity in sheep Georges.pdfVIP

L E T T E R S A mutation creating a potential illegitimate microRNA s target site in the myostatin gene affects muscularity c i t e n in sheep e g e r u t 1,6 1,6 1,6 1,6 1 ´2 a Alex Clop , Fabienne Marcq , Haruko Takeda , Dimitri Pirottin , Xavier Tordoir , Bernard Bibe , n / 2 1 2 2 2 3 m Jacques Bouix , Florian Caiment , Jean-Michel Elsen , Francis Eychenne , Catherine Larzul , Elisabeth Laville , o 1 4 5 1 1 c Franc¸oise Meish , Dragan Milenkovic , James Tobin , Carole Charlier Michel Georges . e r u t a n . w Texel sheep are renowned for their exceptional meatiness. breeds (Supplementary Table 1 online). The confidence interval (c.i.) w w To identify the genes underlying this economically important for the QTL spanned 10 cM (Fig. 1a). ‘Within-family’ analyses showed / / : feature, we performed a whole-genome scan in a Romanov that the three F1 rams were heterozygous TR (Supplementary Fig. 1 p t t Texel F2 population. We mapped a quantitative trait locus online). A QTL with similar effects and location has been detected in h p with a major effect on muscle mass to chromosome 2 and other Texel-based pedigrees4. u subsequently fine-mapped it to a chromosome interval To refine the map position of this QTL we generated a higher- o r G encompassing the my