医学论文：食管癌术前五倍子对表皮葡萄球菌的抗菌作用及其形态学变化.docVIP

??????????????? 作者：李仲兴,王秀华,孟晓洁,杨敬芳,时东彦,王鑫【摘要】? 目的 观察五倍子乙醇提取物对表皮葡萄球菌的体外抗菌活性及其形态学变化。方法 采用新的中药抑菌实验方法对五倍子乙醇提取物进行124株表皮葡萄球菌的最低抑菌浓度测定,同时用显微镜观察用药后菌细胞形态和结构改变。结果 五倍子乙醇提取物对43株耐甲氧西林的表皮葡萄球菌(mrse)和81株甲氧西林敏感的表皮葡萄球菌(msse)的mic90分别为0.288、0.144 mg/ml；在显微镜下,经五倍子处理的表皮葡萄球菌菌细胞逐渐膨胀、增大,可能是五倍子进入菌细胞后,其抗菌物质与菌细胞发挥杀菌作用所致。膨胀到一定大小时细胞内含物逐渐消失。结论 五倍子乙醇提取物对表皮葡萄球菌具有较强的抗菌作用,可使菌细胞形态发生显著改变。 【关键词】? 五倍子；乙醇提取物；耐甲氧西林表皮葡萄球菌；甲氧西林敏感表皮葡萄球菌；细菌形态　　abstract：objective to evaluate the antibacterial activity in vitro of galla chinensis ethanol extractant (gcee) against s. epidermidis, and the change of cell morphology of s. epidermidis. methods deterimination of antibacterial activity of gcee against 124 strains s. epidermidis was performed by using agar dilution method, and the changes of cell merphalogy of s. epidermidis was examined by microscope. results the mic90 of gcee against 43 strains of mrse (methicillin-resistant staphylococcus epidermidis) and 81 strains of msse (methicillin-sensitive staphylococcus epidermidis) were 0.288 and 0.144 mg/ml respectively. under microscope, the cells of s. epidermidis were expansion by degrees, with the edge of cell of s. epidermidis irregular. the? substances in cells of s. epidermidis were disappear final. conclusion galla chinensis ethanol extractant had strong antibacterial activity against 124 strains of s. epidermidis, and the cell morphology of s. epidermidis changed.??? 　　key words：galla chinensis；ethanol extractant；methicillin-resistant staphycoccus epidermidis；methicillin-sensitive staphycoccus epidermidis；bacterial morphology?　　在对抗感染中药的抑菌实验筛选中,发现五倍子对表皮葡萄球菌有极强的抑菌作用1。为了更好的发挥抗感染中药的抗菌作用,为临床应用打下基础,笔者将五倍子乙醇提取物进行了124株葡萄球菌的最低抑菌浓度测定,并用电镜观察五倍子对表皮葡萄球菌超微结构影响的同时,观察五倍子对表皮形态和结构的影响。现报道如下。　　1? 实验材料　　1.1? 菌种准备　　从本院临床患者的血液、尿液、脓汁、痰等标本分离的表皮葡萄球菌共236株,均经api系统(法国生物梅里埃公司生产)及参照manual for clinical microbiology等进行鉴定2。鉴定后按nccls(2002年版)标准确定耐甲氧西林的表皮葡萄球菌(mrse)和甲氧西林敏感的表皮葡萄球菌(msse)。质控菌株为金葡萄球菌atcc 25923,各菌株制成0.5麦氏单位的菌悬液。　　1.2? 仪器　　微量多点接种仪,北京医科大学昆仑药物发展研究所生产。　　1.3? 培养基　　mueller-hinton琼脂(美国bd公司生产),