Highconsistencyenzymatichydrolysisofhardwoodsubstrates翻译.pdfVIP

Bioresource Technology 100 (2009) 5890–5897 Contents lists available at ScienceDirect Bioresource Technology journal homepage: www.elsevier .com/locate/biortech High consistency enzymatic hydrolysis of hardwood substrates Xiao Zhang a,*, Wenjuan Qin b, Michael G. Paice a, John N. Saddler b a FPInnovations Paprican Division (PAPRICAN), 570 boul. St-Jean, Pointe-Claire, Quebec, Canada H9R 3J9 b Faculty of Forestry, University of British Columbia, 2424 Main Mall, Vancovuer, BC, Canada V6T 1Z4 a r t i c l e i n f o a b s t r a c t Article history: The feasibility of using a laboratory peg mixer to carry out high consistency enzymatic hydrolysis of lig- Received 16 September 2008 nocellulosic substrates was investigated. Two hardwood substrates, unbleached hardwood pulp (UBHW) Received in revised form 1 May 2009 and organosolv pretreated poplar (OPP), were used in this study. Hydrolysis of UBHW and OPP at 20% Accepted 20 June 2009 substrate consistency led to a high glucose concentration in the final hydrolysate. For example, a 48 h Available online 29 July 2009 enzymatic hydrolysis of OPP resulted in a hydrolysate with 158 g/L of glucose. This is the highest glucose concentration ever obtained from enzymatic hydrolysis of lignocellulosic substrates. Fermentation of Keywords: UBHW and OPP hydrolysates with high glucose content led to high ethanol concentrations, 50.4 and