花后高温对小麦籽粒氮代谢关键酶活性的影响.docVIP

  花后高温对小麦籽粒氮代谢关键酶活性的 影响# 王晨阳1,2，卢红芳1，马英2，王永华2，尹云星1* 5 10 15 20 25 30 （1. 河南农业大学农学院，河南郑州 450002； 2. 国家小麦工程技术研究中心，河南郑州 450002） 摘要：为探讨花后高温胁迫对小麦籽粒氮代谢及蛋白质合成的影响机制，采用盆栽并利用人 工气候室模拟花后高温的方式，研究了灌浆前期短暂高温对郑麦 366 强势粒和弱势粒 GPT （谷丙转氨酶）、GOT（谷草转氨酶）活性的影响。结果表明，灌浆前期高温显著提高了籽 粒蛋白质相对含量，降低了籽粒蛋白质积累量和氮代谢关键酶 GPT、GOT 活性，其中强势 粒 GPT 活性受高温胁迫的影响较大。小麦籽粒蛋白质相对含量与 GPT 和 GOT 活性均呈负 相关关系，但相关性大小在不同时期、强势粒和弱势粒之间存在有差异，强势粒 GPT 活性 与蛋白质含量的关系更密切。蛋白质含量增加与淀粉积累下降、粒重降低直接相关。 关键词：郑麦 366；强势粒；弱势粒；GPT 和 GOT 活性；蛋白质含量 中图分类号：请查阅《中国图书馆分类法》 Effects of heat-shock on activities of key enzymes involving in nitrogen metabolism in Wheat Grains in the early stage of grain filling WANG Chenyang1,2, LU Hongfang1, MA Ying2, WA Yonghua2, YIN Yunxing1 (1. College of Agriculture, Henan Agricultural University, Zhengzhou, Henan 450002, China; 2. National Engineering Research Center for Wheat, Zhengzhou, Henan 450002, China) Abstract: Effects of heat-shock in the early stage of grain filling on activities of key enzymes related to nitrogen metabolism in superior and inferior grains of wheat was evaluated by pot experiment combining with the climate-controlled greenhouse. Heat treatment significantly increases the protein concentration, while decreases the protein accumulation and the activities of GPT and GOT. The activities of GPT in superior grains are more sensitive to heat stress. The increase of protein concentration is directly related with the reduction of starch concentration and the grain yield. Significant negative correlation exists between protein concentration and transaminase activities in both superior and inferior grains, although the values are much different among grain filling stages and between different grain positions. GPT activity in superior grains is more closely associated with the protein concentration than in inferior grains. Key words: Zhengmai 366, Superior grain, Inferior grain, GPT and GOT activities, Protein concentration. 35 0 引言 蛋白质是影响小麦品质的重要因素，不仅受小麦遗传特性控制，还受温度和水分等环境 条件的影响[1]。研究表明，土壤干旱和渍水均会改变小麦籽粒中蛋白质含