大白菜-结球甘蓝单体异附加系小孢子再生植株的获得与鉴定.doc

  大白菜—结球甘蓝单体异附加系小孢子再 生植株的获得与鉴定# 徐伦，王彦华，轩淑欣，李晓峰，申书兴** 5 10 15 20 25 30 35 40 （河北农业大学园艺学院，河北 保定 071001） 摘要：为创建大白菜—结球甘蓝易位系，以部分大白菜—结球甘蓝单体异附加系（AA+C1～ AA+C8）为试材，进行了游离小孢子培养研究，每个材料均获得了胚状体，其中 AA+C3、 AA+C4、AA+C5、AA+C7、AA+C8 获得了再生植株， 对获得的部分小孢子植株进行了染色 体数目鉴定和田间性状调查。结果表明，大白菜—结球甘蓝单体异附加系小孢子胚的发生率 显著低于大白菜亲本‘85-1’，且不同附加系小孢子胚的发生率存在显著差异，其中产胚率最 高的 AA+C4平均每花蕾出胚达 32.36 个，而产胚率最低的 AA+C2平均每花蕾产胚量仅为 0.64 个，两者相差近 51 倍。对小孢子植株的染色体数目鉴定结果显示，自然加倍率达 90%以上。 通过对染色体数目为 20 条的 AA+C4、AA+C5 小孢子植株进行性状调查后发现，与亲本大白 菜‘85-1’相比较，部分小孢子植株的性状表现有不同程度的差异。 关键词：大白菜；结球甘蓝；异附加系；小孢子培养；再生植株 中图分类号：S634.1 Regeneration and identification of microspore culture plants from Chinese cabbage-cabbage monosomic alien addition lines XU Lun, WANG Yanhua, XUAN Shuxin, LI Xiaofeng, SHEN Shuxing (College of Horticulture, Agricultural University of Hebei, HeBei BaoDing 071001) Abstract: For creating the translocation lines, the microspore culture of Chinese cabbage- cabbage monosomic alien addition lines（AA+C1～AA+C8）was carried out in the present study. The results showed that the embryos produced from all the tested addition lines, in which plantlets formed from the embryos of AA+C3，AA+C4，AA+C5，AA+C7 and AA+C8 . However, the frequencies of embryogenesis of the alien addition lines were significantly lower than their Chinese cabbage parent ‘85-1’ and different among the alien addition lines. The addition line AA+C4 had the highest embryogenesis frequency, about 32.36 embryos per bud, while the AA+C2 had the lowest, only 0.36 embryos per bud, and the former was nearly 51 times higher than the latter . The chromosome number examination of the plantlets showed that chromosome spontaneous doubling rate was more than 90%. Through surveying the field characters of the AA+C4，AA+C5 microspore plantlets with 20 chromosomes, we found that part of the plantlets had different degree of difference comparing to their Chinese cabbage parent ‘85-1’. Keywords: Chinese cabbage; cabbage; alien addition lines; microspore culture; plant regeneration 大白菜（Bras