肝癌血清中岩藻糖结合蛋白的分离与鉴定.docVIP

  肝癌血清中岩藻糖结合蛋白的分离与鉴定# 党刘毅，李铮** （西北大学功能糖组学实验室， 西安 710069） 5 10 摘要：[目的] 分离并鉴定肝细胞癌血清中的岩藻糖结合蛋白，并为新的癌症诊断标志物的 发现提供新思路。[方法] 利用岩藻糖功能化的磁性微粒进行岩藻糖结合蛋白的分离，并利 用 LC-MS/MS 进行鉴定。[结果] 健康人血清中分离并鉴定岩藻糖结合蛋白 49 种，肝癌患 者血清中分离并鉴定岩藻糖结合蛋白 45 种，其中，健康人与肝癌患者血清同时出现的蛋白 为 26 种。通过非标记定量方法 emPAI 定量比较，有 8 种蛋白在肝癌中低表达，3 种高表达。 [结论] 本研究分离并鉴定了健康人和肝癌患者血清中的岩藻糖结合蛋白，同时，肝癌中高 表达的糖结合蛋白可以作为肝癌诊断的潜在标志物。 关键词：生物化学与分子生物学；肝癌；标志物；岩藻糖；糖结合蛋白 中图分类号：Q513 15 Isolation and Identification of Fucose-binding Proteins in Sera of Hepatocellular Carcinoma DANG Liuyi, LI Zheng (Laboratory of Functional Glycomics, Northwest University, Xian 710069) 20 25 30 35 40 Abstract: Current biomarkers used clinically still suffer from poor sensitivity and specificity in the diagnosis of hepatocellular carcinoma (HCC). Therefore, new strategy is still needed for discovery of biomarker in HCC. Methods: Here a strategy was performed for the isolation and identification of fucose-binding proteins from the clinical sera (60 normal donors and 60 hepatocellular carcinoma patients) by the fucose-magnetic particle conjugates-based method and LC-MS/MS analysis. Results: 75 proteins for normal donors and 79 proteins for HCC cases were identified, of which 26 were identified in both samples. With the exponentially modified Protein Abundance Index (emPAI) quantification, 8 fucose-binding proteins were estimated down-regulated (emPAI ratio 2) and 3 MBPs were estimated up-regulated (emPAI ratio 0.25) in the HCC sera. Conclusions: Fucose-binding proteins were identified as up–regulated in the HCC could be served as potential biomarker and this research also provided new strategy for the discovery of new biomarkers. Key words: Biochemistrty and molecular biology; HCC; Biomarker; Fucose; Glycan-binding protein 0 引言 原发性肝癌是临床上最常见的恶性肿瘤之一。由于起病隐匿，早期没有症状或症状不明 显，进展迅速，确诊时大多数患者已经达到局部晚期或发生远处转移，治疗困难，预后很差。 目前，临床上应用的肝癌诊断标志物，主要为甲胎蛋白（α-fetoprotein），诊断灵敏度和 [1] 蛋白质组学已经成为发现癌症诊断标记物的有力工具。过去十年，血清和血浆是蛋白质 9 [2] 基金项目：教育部高等学校博士学科点专项科研基金（200806970018） 作者简介：党刘毅(1986-),男, 硕士研究生,主要