纳米羟基磷灰石_聚氨酯复合多孔支架的制备与生物相容性.docVIP

  Characterization and Cytocompatibility of Nano-hydroxyapatite/glyceride of Castor Oil Based Polyurehane Scaffolds# 5 10 15 20 25 30 35 40 DU Jingjing, ZOU Qin, LI Yubao** (Research Center for Nano Biaomaterials,Analytical and Testing Center,Sichuan University, Chengdu 610064,P.R.China) Abstract: In this paper，The PU scaffold was fabricated with aliphatic isophorone diisocyanate (IPDI) as hard segment and glyceride of castor oil as soft segment. The n-HA/PU composite scaffolds were developed by in situ polymerization. The obtained PU and n-HA/PU composite scaffolds were characterized by FTIR, and SEM. The mechanical property and porosity were tested. The results showed that the characteristic absorption peak of n-HA and PU were presented in FTIR spectra of n-HA/PU composites. The porosity of PU, n-HA/PU composite with 20 wt% n-HA and n-HA/PU composite with 40 wt% n-HA were 61±3%, 68±2% and 57±3%, respectively. The compressive strength of above three porous scaffolds was 605±61 KPa, 2125±58 KPa and 4588±257 KPa, respectively. To evaluate the cytocompatibility of porous scaffolds, the MG63 cells were seeded on scaffolds. The scaffold contained n-HA were more helpful for cell adhesion, spread and proliferation in 3D cultivation. It is believed that n-HA/PU composite porous scaffolds developed in this study have the potential to be applied in bone reconstruction and repair. Key words: porous scaffold for tussiue engineering ； polyurethane ； nano-hydroxyapatite ； cytocompatibility 0 Introduction The main aim of bone tissue engineering is therefore to clinical application for treatment of bone defect or necrosis. An important component for bone repair is the three-dimensional composite that consists of seed cells and scaffold. Because the natural bone tissue is composed of hydroxyapatite (HA) and organic matrix (type Ⅰ collagen), therefore, the current study is focused on hydroxyapatite/polymer composite in bone tissue engineering. Both natural and synthetic polymer