微酸性电解水对灰葡萄孢菌的杀灭效果研究.docVIP

  微酸性电解水对灰葡萄孢菌的杀灭效果研 究# 冀盈盈，李保明，郝晓霞，曹薇** 5 10 15 20 25 30 35 40 （中国农业大学农业部设施农业工程重点实验室） 摘要：植物病害是引起植物品质下降和产量损失的主要原因之一。农药的滥用或不当使用， 带来了严重的生态环境和食品安全问题。由灰葡萄孢菌（Botrytis cinerea Pers.: Fr.）引起的 植物灰霉病是一种危害性很大的病害。本文旨在将微酸性电解水高效、无污染、无残留和安 全的杀菌特性替代化学农药应用于植物病害防治。主要研究了微酸性电解水（pH 6.0-6.5, ORP 750-850 mV, 有效氯浓度 21-59 mg/L）对灰葡萄孢菌的杀灭效果。考察了有效氯浓度、 杀菌时间和处理温度对微酸性电解水杀菌效果的影响。结果表明：微酸性电解水对灰葡萄孢 菌具有较强的杀灭效果。有效氯浓度超过 31 mg/L 时，处理 30 s，杀菌率可以达到 99%以上； 在 4、22 和 45下有效氯浓度为 40 mg/L 的微酸性电解水可使灰葡萄孢菌分别降低 2.18、 2.88 和 4.72 log10 CFU/mL。 关键词：微酸性电解水；灰葡萄孢菌；杀菌 中图分类号：S129 Inhibition of Botrytis cinerea Pers.: Fr. by slightly acidic electrolyzed functional water JI Yinging, LI Baoming, HAO Xiaoxia, CAO Wei (Key Laboratory of Structure and Environment in Agricultural Engineering, Ministry of Agriculture, China Agricultural University) Abstract: Plant diseases are the main cause of deterioration of the quality and yield losses of plant. Misusing or improper using the pesticide has brought the severe environmental and food safety issues. The gray mold disease caused by Botrytis cinerea is a great threat to plant production. The purpose of the present study was to investigate the application of slightly acidic electrolyzed functional water (SAEFW) with strong bactericidal or fungicidal activity, no pollution and no residues instead of chemical pesticides in plant protection. The fungicidal efficiency of SAEFW with pH values of 6.0-6.5, oxidation-reduction potential (ORP) of 750-850 mV and available chlorine of 21-59 mg/L for inactivating the Botrytis cinerea Pers.: Fr. was examined. The effect of available chlorine concentration, treatment time and temperature on the fungicidal activity of SAEFW was also determined. The results indicate that SAEFW had powerful fungicidal activity for inhibition of Botrytis cinerea Pers.: Fr. A 99% of Botrytis cinerea Pers.: Fr. was killed by SAEFW at an available chlorine concentration of 31 mg/L for 30 s. The population of Botrytis cinerea Pers.: Fr. was reduced 2.18, 2.88 and 4.7