肾发育中增殖相关蛋白的表达与凋亡细胞的分布.docVIP

  肾发育中增殖相关蛋白的表达与凋亡细胞 的分布# 宋小峰1,2，任昊1，梁飞1，翟效月1** 5 10 15 20 25 30 35 （1. 中国医科大学组织学与胚胎学教研室，沈阳 110001； 2. 辽宁医学院组织学与胚胎学教研室，辽宁 锦州 121001） 摘要：目的：本研究通过传统的和改良的组织化学方法显示生后 14 天小鼠肾增殖相关蛋白 Ki67 和凋亡细胞的分布。方法：取生后 14 天的小鼠，经灌流固定后，取双侧肾组织，分别 制备石蜡和树脂 812 包埋的组织块。石蜡切片 5μm 厚，树脂切片 2.5μm 厚。在石蜡切片上 进行传统的免疫细胞化学染色，而在树脂切片上进行改良的免疫细胞化学染色，随后检测增 殖相关蛋白 Ki67 和凋亡阳性细胞（Tunel 染色）的表达。结果：树脂切片与石蜡切片上 Tunel 阳性细胞的表达一致，分布比较广泛；但 Ki67 只在石蜡切片制备的肾组织上特异性的表达 在髓放线的小管细胞核上。结论：树脂切片上进行免疫组化虽然能在特异性的显示组织蛋白 的同时展示较好的组织结构，但是其免疫组化反应仍有局限性。生后肾发育时凋亡现象较普 遍，增殖相关蛋白主要表达在近端小管细胞核上。 关键词：树脂切片；肾脏；Ki67；Tunel；小鼠 中图分类号：R329.1 The expression of proteins relating to proliferation and the distrussion of apoptotic cells of renal development SONG Xiaofeng1,2, REN Hao1, LIANG Fei1, ZHAI Xiaoyue1 (1. Deparment of Histology and Embryology, China Medical University, Shenyang 110001; 2. Deparment of Histology and Embryology, Liaoning Medical College, LiaoNing JinZhou 121001) Abstract: Objective: This research demonstrated the distribution of mice kidney proliferation related proteins Ki67 and the apoptotic cells after p14d by traditional and modified organization chemical methods. Methods: After 14 days of mices birth, we took their bilateral renal organization after their bodys were perfusion fixed, to make epoxy and resin 812 embedding tissue mass, and make epoxy section 5μm and resin slice 2.5μm in thick. Next step, the resin slice was processed by traditional immunecytochemistry dyeing, and the epoxy section was processed by improved immunecytochemistry dyeing, afterward we detect expression of Ki67 and apoptosis positive cells. Result: The expression of apoptotic cells both on epoxy and resin section were consistent, and have more extensive distribution, while Ki67 was specifically expressed only on the kidney tissues made from resin section and only expressed on tubule nucleus processed by medullary ray. Conclusion: Although immunohistochemistry on resin slice can display in specific tissue protein and show better organization structure, but it