四个miRNA在鸡性分化早期的性别差异表达谱分析.docVIP

  四个 miRNA 在鸡性分化早期的性别差异表 达谱分析# 王晓，苗楠，龚炎长，俸艳萍** 5 10 15 20 25 30 35 40 （华中农业大学农业动物遗传育种与繁殖教育部重点实验室，武汉 430070） 摘要：miRNA 是一类长度约为 20～24 个核苷酸的小分子非编码 RNA，它通过调控靶基因 转录后的表达而在多种生物学途径中发挥作用。为探讨 miRNA 在性腺早期发育中的作用， 本课题组在前期通过基因芯片方法筛选出了在 E3.5～6.5 天雌、雄鸡胚中呈差异表达的若干 个 miRNAs，本研究即采用茎环状引物的定量 PCR 方法对其中 4 个 miRNAs 进行表达谱分 析。结果表明，miR-107、1767 这 2 个 miRNAs 呈现前期雌性高表达后期雄性高表达的趋势， miR-2954 的表达则是在 E4.5d 雄性中极显著高于雌性(P0.01)，miR-92 在所检测的各时期（除 E4.5d 外）中的表达均为雄性极显著高于雌性 (P0.01)。本文根据表达谱结果推测了 miR-92 可能涉及早期鸡胚性腺发育过程。 关键词：鸡；miRNA；性腺发育；定量 PCR 中图分类号：S831.2 The sexually dimorphic expression profile analysis of four miRNAs in chicken embryo at the early stages of sex diferentiation Wang Xiao, Miao Nan, Gong Yanzhang, Feng Yanping (Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan 430070) Abstract: miRNA is a family of small, non-coding RNA molecules of about 20-24 nucleotides, which regulate many biological pathways by down-regulate target gene expression at the post-transcriptional level. In order to investigate the function of miRNA on the early development of chicken gonad., some sexually dimorphic miRNA expressions during E3.5~6.5 in chicken embryos had been found by miRNA chip in previous studies. In this study, expression patterns of 4 miRNAs which selected based on the early study were studied by stem-loop Quantitative PCR methods. The results showed miR-107 and 1767 were higher expression in female during early stage, but higher in male during later stage; miR-2954 was with significant higher expression in male at E4.5d(P0.01), and miR-92 was expressed at significant higher level in male at all detected stages, except for E4.5d (P0.01). Based on the expression results, it is speculated that miR-92 should have effected on the early process of gonad development in chicken embryo. Key words: chicken; miRNA; gonad development; Quantitative PCR 0 引言 MicroRNA（miRNA）是一类生物体内长约20~24nt的单链非编码小分子RNA，通过与 其靶mRNA 3’端非翻译区（3’UTR）完全或