金纹细蛾几丁质酶基因生物信息学分析.docVIP

  金纹细蛾几丁质酶基因生物信息学分析# 范晓军，宋志芳，仙笑笑，李瑶，赵秋勇** （太原理工大学化学化工学院生物与制药工程系，太原 030024） 5 10 摘要：本文从生物信息学角度对克隆获得的金纹细蛾几丁质酶进行分析，通过课题组提交到 GenBank 的数据，采用在线分析及相应软件分析预测金纹细蛾几丁质酶(LrCHI)基因核苷酸 和氨基酸序列的组成、理化性质、信号肽、糖基化位点、磷酸化位点、疏水性、二级结构和 三级结构等，并构建系统发育树。结果表明：LrCHI 开放阅读框由 1737 bp 组成，推导 578 个氨基酸；此序列所编码的蛋白属于几丁质酶 18 家族，其 N 端含有信号肽和几丁质酶 18 家族活性位点，C 端为几丁质结合区，无跨膜结构域区域；预测 LrCHI 为亲水性蛋白；金纹 细蛾与鳞翅目的棉铃虫、甜菜夜蛾进化关系最近。分析结果可为 LrCHI 的科学研究提供有价 值的信息，为进一步研究其高级结构与功能的关系提供理论依据。 关键词：金纹细蛾；几丁质酶；生物信息学分析；理化性质；系统发育树 中图分类号：Q781 15 Bioinformatical analysis of chitinase gene from Lithocolletis ringoniella FAN xiaojun, SONG Zhifang, XIAN Xiaoxiao, LI Yao, ZHAO Qiuyong (Department of Biological and Pharmaceutical Engineering, College of Chemical Engineering and 20 25 30 35 40 Technology, Taiyuan University of Technology, TaiYuan 030024) Abstract: In this paper we analyzed Lithocolletis ringoniella chitinase from the perspective of bioinformatics. Based on the date that has been submitted to GenBank by our group, Nucleotide sequence and deduced amino acid sequence of Lithocolletis ringoniella chitinase were analyzed and predicted by the tools of bioinformatics which are online or corresponding software in the following aspects: the composition, physical and chemical properties, signalpeptide, O-glycosylation site, Phosphorylation site, Hydrophobic character, secondary structure, tertiary structure,etc. We also build phylogenetic trees. The results showed that the open reading frame (ORF) of LrCHI consistes of 1737 bp which deduces 578 amino acid sequences. The protein coded by this sequence belongs to chitinase family 18. There are a signal peptide and a chitinase family 18 active site in the N-terminal region of the polypeptide chain. There is a chitin-binding site in the C-terminal region. No possible transmembrane domain region. LrCHI may be a hydrophilic protein. Lithocolletis ringoniella, phylogenetic relationship is the nearest to Helicoverpa armigera and Spodoptera exigua than other insects . The result of this study