灵芝和金针菇混和发酵提高锌产量的工艺研究.pdfVIP

现代食品科技 Modern Food Science and Technology 2013, Vol.29, No.2 灵芝和金针菇混和发酵提高锌产量的工艺研究 苗敬芝，董玉玮，耿治飞 （徐州工程学院食品工程学院，江苏省食品资源开发与质量安全重点建设实验室，江苏徐州 221111） 摘要：本实验采用混菌发酵技术，以信州灵芝和金针菇为出发株，探讨灵芝、金针菇混菌发酵高产锌元素的工艺条件，采用原子 吸收分光度法测定胞内锌元素含量。结果表明：最佳培养基为 2% 山芋淀粉、2%马铃薯、1%蔗糖、2%玉米粉、1%黄豆粉；混菌发酵 最佳培养条件为培养温度27 ℃，转速 150 r/min，装液量 100 mL/250 mL，培养时间8 d；在最佳条件下，混菌发酵胞内锌元素含量为 45.6 mg/kg ，比灵芝单独培养提高 15.8%，比金针菇单独培养提高63.5%。说明混菌发酵更完全，对培养基中的营养物质吸收利用率 高，菌丝体长势好，增加了胞内锌产量。 关键词：灵芝；金针菇；混菌发酵；锌元素 n 文章篇号：1673-9078(2013)2-287-290 c . Mixed Fermentation of G.lucidum XinZhi and Flammulina velutipes for d High-yield Zinc Production o MIAO Jing-zhi, DONG Yu-wei, Geng Zhi-fei o (College of Food (Biology) Engineering, Xuzhou Institute of Technology, Key Construction Laboratory of Food Resource f Development, Quality and Safety of Jiangsu Province, Xuzhou 221111, China) n Abstract: G.lucidum XinZhi and Flammulina velutipes were used as the original strains for mixing fermentation to discuss technology r conditions of high-yield zinc. Quality of zinc from mycelium was determined by atomic absorption spectrum method. The results showed that e the best medium was 2% starch, 2% potato, 1% sucrose, 2% corn meal, and 1% soybean meal. The optimal fermentation conditions were cultured time 8 days, temperature 27 ℃, shaking speed 150 r/min, and medium volume 100 mL/250mL. Quality of intracellular zinc was 45.6