三角帆蚌寒凉药性与多糖效应相关性研究[J].docVIP

三角帆蚌寒凉药性与多糖效应相关性研究 Correlation Study on Cold Property of Hyriopsis Cumingii and Effect of Polysaccharides 陈文星 吴 皓1 陆 茵1、2 李利斌3 CHEN Wenxing1 WU Hao1 LU Yin1，2 LI Libin3 南京中医药大学中医药研究院（中国 210029）1 Chinese Medicine Institute of Nanjing University of Chinese Medicine（China 210029）1 江苏省方剂研究重点实验室（中国 210046）2 Jiangsu Provincial Key Laboratory for Fangji Research（China 210046）2 徐州市中医院神经内科（中国 221000）3 Traditional Chinese Medical Hospital of Xuzhou（China 221000）3 中图分类号：R28 文献标识码：A 文章编号：1818－0086（2008）02 摘 要：目的 实验研究多糖效应，与三角帆蚌寒凉药性联系比较，分析二者的相关性。以ADP和PAF为诱导剂，测定血小板最大聚集率；建立大鼠静脉注射内毒素（10u·kgP-1P）诱导的实热模型和附子、肉桂、干姜合剂灌服大鼠造成的虚热模型，分别观察多糖对血小板聚集和热证模型的影响。多糖100(g·mlP-1P、50(g·mlP-1体外明显促进血小板聚集；静脉分别注射140mg·kgP-1P、70mg·kgP-1 30min后P对内毒素引起的温度升高有明显的降低作用（P 0.05）；140mg·kgP-1P可明显降低虚热模型大鼠的血清T3值（P 0.05）。多糖表现明显的促凝集作用和解热作用，体现了寒凉的特性，三角帆蚌寒凉药性与多糖有明显的相关性，多糖是三角帆蚌寒凉药性的物质基础。 三角帆蚌；寒凉药性；多糖；促凝集作用；解热作用；药性物质基础 analyzing relationship between effect of polysaccharides from mussels and cold drug-property of mussels’ soft body. Methods maximum agglutination induced by ADP and PAF was assayed；two types of rat heat-syndrome model induced respectively by injection of endotoxin or by administration of decoction composed of Fuzi, Rougui and Ganjiang were used. polysaccharides from mussels should activate the platelet in vitro. Polysaccharides of 140 mg·kgP-1 and 70 mg·kgP-1 in vein indicate significantly antipyretic effect and heat syndrome due to insufficiency of Yin fluids in rats was disappeared through decreasing T3 level of serum. Conclusion polysaccharides from mussels whose antipyretic effect and promotion of coagulation indicates cold property is the basic substance. Key words: soft body of mussels; cold property; polysaccharides; antipyretic effect; promotion of coagulation; basic substance for drug-property 蚌科动物三角帆蚌（Hyriopsis cumingii）用于培养珍珠的动物软体部分，即蚌肉，其性寒、味甘咸，入肝、肾二经。其入药在中医药理论文献中也早有记载。《日华子本草》：“蚌，明目，止消渴，除烦解热毒，补妇人虚劳、下血，并痔痿、血崩、带下”；《本草再新》：“治肝热，肾衰，托斑疹，解痘毒，清凉止渴”。 从三角帆蚌软体的水煎粗提物中经过多次分离初次得到一多糖，经分析为均