端粒酶活性检测联合癌胚抗原, CAl9—9 对良恶性胸水的鉴别诊断价值.pdfVIP

· 197· ．论著． 端粒酶活性检测联合癌胚抗原、CAl9—9对良恶性 胸水的鉴别诊断价值 王佩芳 【摘要】目的探讨端粒酶活性联合癌胚抗原(CEA)、CAl9—9在鉴别良、恶性胸水中的价值。方法 入选58例胸水患者，用改良的端粒重复序列扩增一酶联免疫吸附试验法检测胸水中脱落细胞端粒酶活 性，并测定胸水CEA、CAl9-9。结果恶性胸水中端粒酶活性检出阳性率为85．7％。恶性胸水患者中端 粒酶活性、CEA、CAl9-9测定结果均明显高于良性胸水患者(Po．01)。联合端粒酶、CEA、CAl9—9共 同检测，则敏感性为0．971，特异性为I．000。结论端粒酶在诊断恶性胸水和鉴别良、恶性胸水中具有重 要的价值，联合CEA、CAl9-9对良、恶性胸水鉴别诊断意义更大。 【关键词】端粒酶；癌胚抗原；CAl9—9；胸水；鉴别诊断 ofteiomerasecombinedwith CAl9-9for Diagnostic and significance activity carcinoembryonicantigen and effussion WANG differentiatingbenignmalignantpleural Pei—fang．DepartmentofRespiratory Cixi 315300，China Medicine，People’sHospitalof City，Gxi [Abstract] To the oftelomerasecombined Objectiveinvestigatediagnosticsignificance activity with CAl9—9／or and carcinoembryonieantigen(CEA)anddifferentiatingbenignmalignantpleural Telomerase andCAl9—9in fromcaseswere effussion．Methodsactivity。CEA effussion58 pleuI=al detectedthe of telomeric linked by technique repeatamplification fmproved protocol—enzyme immunosorbent The rateoftelomerasein effussionwas positive assay．Results activitymalignantpleural telomerase andCAl9—9in effussionwere 85。7％．The activity，CEA malignantpleural significantlyhigher thosein of than effussion(P0．01)．Theand telomerase benignpleural sensitivityspecifi