免疫磁珠分选和MLPA 技术联合检测多发性骨髓瘤分子遗传学异常系统的建立.pdfVIP

·1332 · 南方医科大学学报（J South Med Univ ） 2012;32(9) 临床研究 免疫磁珠分选和MLPA 技术联合检测多发性骨髓瘤分子遗传学 异常系统的建立 1 2 3 3 3 3 3 雷 鸣 ，马 莉 ，邝丽萍 ，庞 妍 ，伍九龙 ，肖芷芳 ，李 力 1 2 3 广州军区广州总医院 肾脏内科，体检中心，血液科，广东 广州 510010 摘要：目的探讨联合免疫磁珠分选和多重探针连接依赖性扩增（MLPA）技术检测多发性骨髓瘤（MM）分子遗传学异常的可靠 性。方法 收集29 例初诊MM 患者的骨髓细胞，用CD138磁珠进行分选，设计MLPA 探针检测分选前后标本TP53 和RB 1 的表 达情况，并与FISH 检测结果进行对照。结果 MLPA 检测成功率100% ，与FISH 结果吻合度达99.1% ，分选后MLPA 和FISH 阳 性率均有显著性提高。结论 MLPA 适合临床MM 分子遗传学异常检测，标本应在检测前进行磁珠分选。 关键词：多发性骨髓瘤；多重连接依赖性扩增；荧光原位杂交；免疫磁珠分选 中图分类号：R738.1 文献标志码：A 文章编号：1673-4254（2012）09-1332-04 doi: 10.3969/j.issn. 1673-4254.2012.09.027 /kcms/detail/44.1627.R0923.011.html Magnetic-activated cell sorting combined with multiplex ligation-dependent probe amplification for detecting molecular cytogenetic abnormalities in multiple myeloma 1 2 3 3 3 3 3 LEI Ming , MA Li , KUANG Liping , PANG Yan , WU Jiulong , XIAO Zhifang , LI Li 1Department of Nephrology, 2Medical Health Center, 3Department of Hematology, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010, China Abstract: Objective To investigate the reliability of magnetic-activated cell sorting (MACS) combined with multiplex ligation-dependent probe amplification (MLPA) in the detection of the molecular cytogenetic abormalities in multiple myeloma. Methods The bone marrow cells were colelcted from 29 patients with multiple myeloma. The immunomagnetically sorted and unsorted cells were detected for TP53 and RB1 expressions using MLPA probes and the results were compared with fluorescent in situ hybridization (FISH). Results The detection success rate was 100% for MLPA, which yielded results with an concordance rate