丙泊酚对大鼠肠缺血再灌注损伤时肝细胞凋亡及Caspase-3 的影响.docVIP

丙泊酚对大鼠肠缺血再灌注损伤时肝细胞凋亡及Caspase-3的影响 E-mail ：cdr128@126.comCaspase-3的影响。方法 成年健康Wistar大鼠42只，随机分为3组：实验对照组(C组,n=6)、肠缺血再灌注组(IR组,n=18)、丙泊酚干预组(P组，n=18)。各组根据再灌注时间分为1，3，6h三个时相。通过夹闭肠系膜上动脉制作肠缺血再灌注损伤模型，实验结束后即刻取肝左叶做标本。用免疫组化法检测Caspase-3蛋白表达量，TUNEL法检测肝细胞凋亡指数（AI）。结果 与C组比较，IR组和P组肝组织Caspase-3蛋白含量、AI均增加（P 0.05）；与IR组比较， P组Caspase-3蛋白表达减少、AI减少（P 0.05）大鼠肠缺血再灌注时Caspase-3蛋白表达，使肝细胞凋亡减轻，从而对肝损伤有一定的保护作用。 Caspase-3 Effects of propofol on hepatic cell apoptosis and expression of Caspase-3 with intestinal ischemia-reperfusion injury in rats YIN Yuan，CAO Ding-rui*，YUAN Yuan YANG chun-yan，ZHANG wen-jie (Dept of Anesthesiology，First Clinical Medical College，Shanxi Medical University，Taiyuan 030001，China；*Corresponding author：CAO Ding-rui，E-mail：cdr128@126.com Objective To investigate the effects of propofol on hepatic cell apoptosis and expression of Caspase-3 with intestinal ischemia-reperfusion injury in rats. Methods Forty-two Wistar rats were randomly divided into 3 groups：control group(C group,n=6)，ischemia-reperfusion group(IR group,n=18)，propofol group(P group,n=18).The rats were executed in each group after reperfusion for 1h,3h and 6h respectively.A rat model of intestinal ischemia-reperfusion was established by blocking the superior mesenteric artery with clap．The left hepatic lobes were sampled at the end of reperfusion．Caspase-3 protein expression in liver tissues was assessed by immunohistochemical method. The apoptosis index（AI）of hepatic cell was measured by TUNEL method．Results Caspase-3 protein expression and AI of hepatic cell were significantly higher in IR group and P group than in C group（P 0. 05）．Caspase-3 protein expression and AI of hepatic cell were significantly lower in P group than in IR group（P 0. 05）．Conclusions propofol can relieve the liver cellular apoptosis by decreasing Caspase-3 protein expression with may protect liver from intestinal ischemia-reperfusion injury． Keywords: propofol；Liver；Ischemia-Reperfusion injury；Apoptosis；Caspase-3 肠缺血-再灌注损伤 (intestinal i