青藏高原7种嵩草遗传多样性AFLP研究-中国农业科学学报.docVIP

青藏高原7种嵩草遗传多样性AFLP研究 郑，呼，王1西北农林科技大学陕西杨凌 712100；2陕西省农业分子生物学重实验室，陕西杨凌 712100 of Genetic Diversity in Seven Kobresia by AFLP Tibet Plateau of China ZHENG Hong-mei1, HU Tian-ming2, WANG Quan-zhen2, ZHANG Guo-yun3, SONG Jiang-hu2 (1College of Resourses and Environment, Northwest Agricultural and Forestry University, Yangling 712100, Shaanxi; 2College of Animal Science and Technology, Northwest Agricultural and Forestry University, Yangling 712100, Shaanxi; 3Key Laboratory Molecular Biology of Agricuture Shaanxi, Yangling 712100, Shaanxi) Abstract:【Objective】This work analyzed the genetic diversity of Kobresia accessions, in the molecular level, and further obtained the helpful information for breeding and germplasm evaluation.【Method】Genomic DNA of Kobresia was amplified with four E+3 and M+3 primer combinations with AFLP.【Result】AFLP analysis produced 164 score able bands, among them 154 (93.96%) were polymorphic. The mean Nei’s gene diversity index (H) was 0.2430, the Shannon’s information index (I) was 0.4012, indicating that genetic diversity of Kobresia is abundant. The number of 11 Kobresia accessions from Tibetan Plateau can be classified into five groups with cluster analysis based on the UPGMA method.【Conclusion】In general, there abundant genetic diversity among Kobresia accessions resources, and the genetic coefficient is unrelated to their geographic latitude. Natural habitats influenc genetic differentiation of Kobresia. Key words: Kobresia; Genetic diversity; AFLP 0 引言 【研究意义】嵩草属Kobresia Willd.）植物属莎草科中的苔草亚科，是多年生草本植物，多数是高寒草甸的建群种，它们具有草质柔软、营养丰富等特点，为各类家畜所喜食，是青藏高原控制水土流失，保持生态平衡的重要植物种类[1]。嵩草属植物作为高寒草甸主要的建群种，其研究对促进西藏高寒草地生态建设、改良天然草地、建立人工草地和发展草地畜牧业具有极其重要的科学研究和生产价值。【前人研究进展】AFLP自其问世以来，该方法已被广泛应用于生物多样性研究[4,5]、图谱构建[6,7]、品种鉴定[8,9]、基因定位分离[10,11]、杂种优势预测[12]和分子标记辅助选择育种[13]等多方面的研究，在多种植物如野生鸭茅、羽扇豆、烟草、羊草、玉米等[1418]的研究中已取得很大进展。【本研究切入点】嵩草种质资源的分子标记目前虽做了一些初步研究，但仅限于RAPD分析[2,3]，未见有关嵩草AFLPamplified fragment length polymorphism）分析的报道。【拟解决的关键问题】本试验采用AFLP 分子标记技术对11份青藏高原嵩草的基因组DNA进行多态性扩增，