临床研究H-NMR的模式识别方法在慢性心力衰竭患者血清代谢组.pdfVIP

2012;32(3) 南方医科大学学报（J South Med Univ ） ·415 · 临临床研究床研究 1 基于 H-NMR 的模式识别方法在慢性心力衰竭患者血清代谢组 学中的应用 杜智勇，沈安娜，苏 亮，梁健球，许顶立 南方医科大学南方医院心血管内科，广东 广州 510515 1 1 摘要：目的探讨基于 H-NMR 的模式识别方法应用于慢性心力衰竭（CHF）血清代谢组学研究的可行性。方法 应用 H-NMR 技 术对9 例CHF 患者和6 例健康人血清进行检测，对所得数据分别采用主成分分析法（PCA）和正交信号校正偏最小二乘法 （OPLS）进行模式识别分析，确定CHF 患者与健康人血清差异代谢物，比较两种模式识别方法判别能力。结果 CHF 患者与健 1 康人血清 H-NMR 图谱有明显差异。其中PCA 模式识别法未能将CHF 组和健康对照组区分开，而用OPLS模式识别方法两组 1 可明显区分。结论 H-NMR 技术是研究CHF 代谢组学的有效技术手段，CHF 患者血清代谢组与健康人存在明显差异。OPLS 模式识别法明显优于PCA 法，能够去除非实验因素的影响，提高分类效果。 关键词：慢性心力衰竭；磁共振氢谱；代谢组学；模式识别 中图分类号：R541.6 文献标志码：A 文章编号：1673-4254（2012）03-0415-05 DOI: CNKI:44-1627/R1124.005 /kcms/detail/44.1627.R1124.005.html Application of 1H-NMR-based pattern recognition in serum metabolomics of patients with chronic heart failure DU Zhiyong, SHEN Anna, SU Liang, LIANG Jianqiu, XU Dingli Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China Abstract: Objective To investigate the feasibility of applying 1H-NMR-based pattern recognition in the studies of serum metabonomics in chronic heart failure (HF). Methods 1H-NMR technique was applied for examination of the serum samples from 9 patients with chronic heart failure and 6 healthy individuals. The data were analyzed for pattern recognition through principal component analysis (PCA) and Orthogonal Partial Least Square (OPLS) to determine the differences in serum metabolites between the two groups. The recognition ability of the two analysis methods were compared. Results The serum 1H-NMR spectra of heart failure patients and healthy individuals were significantly di