不同配比的阳离子脂质体对APC成熟活化和抗原摄取功能的影响.pdfVIP

不同配比的阳离子脂质体对APC成熟活化和抗原摄取功能的影响 不同配比的阳离子脂质体对APC成熟 活化和抗原摄取功能的影响 Different Oompositions of Cationic Liposome Regulate the Activation of Antigen-presenting Cell and Antigen Uptake 谢晓芳 周冬梅 庄燕 马轶凡 摘 要 许多研究证明阳离子脂质体作为一种良好的佐剂，可以促进抗原特异性的免疫反应。本研究中，我们合成一系列 的DOTAP与DOPC不同比例组成的阳离子脂质体复合物，研究不同组成比例对脂质体的免疫调节作用的影响。结果显示， DOTAP/DOPC(摩尔比为4:1)阳离子脂质体不但表现出很强的诱导单核细胞和树突状细胞活化的能力，而且可以促进巨噬 细胞对抗原的摄取。因此，我们发现合适比例的DOTAP/DOPC阳离子脂质体可以促进抗原呈递细胞的活化以及增强对抗 原的摄取能力。 关键词 阳离子脂质体；APC；佐剂 ABSTRACT　Cationic liposomes have been shown as a potent adjuvant to promote antigen-specific immune responses. In the present study, we incorporated a serial amount of DOPC into DOTAP liposome, and investigated the immunoregulatory effect of different cationic liposome formulations. The result showed that DOTAP/DOPC with the molar ratio of 4:1 not only significantly induced monocytes activation and dendritic cells maturation, but also enhanced the antigen uptake by macrophage. Hence, cationic liposome with an appropriate ratio of DOTAP to DOPC could enhance the activation of antigen presenting cells and antigen uptake. KEYWORDS Aationic Liposomes APC Adjuvant ； ； 1 Introduction Cationic liposomes are not only an effective delivery of antigen presenting cells (APC) and the capability system for gene and protein vaccines, but also have been of antigen uptake. Our aim is to develop an optimal shown as a potent adjuvant to enhance antigen-specific liposome formulation with remarkable immuno-potential [1,2] immune responses . However, although addition of effect for vaccine delivery. neutral lipids into cationic liposome has been shown to [3] 2 Materials and Methods reduce th