重组乳酸菌作为黏膜免疫呈递载体的研究.pptVIP

How probiotics maymodulate human mucosal responses益生菌与人肠道黏膜可能的作用机制 姓名：赵伟 学号： 2011年12月5日 Background According to the currently adopted definition by FAO/WHO, probiotics are: Live microorganisms which when administered in adequate amounts confer a health benefit on the host. 益生菌是指当摄入足量菌数时能对宿主产生健康益处的微生物活体。 Benefits： 定植和生长所产生的健康作用：调节肠道菌群，与上皮细胞粘附 代谢及产生营养物质促健康作用：合成维生素、有机酸、胞外多糖等 其他：抗菌、缓解腹泻、抗过敏，治疗肠胃炎症等 Probiotic bacteria are proposed to deliver health benefits to the consumer by modulation of intestinal function through largely unknown molecular mechanisms. 益生菌被认为对人体有很多有益作用，但是其对人肠道黏膜作用方式的主要分子机理还不为人知。 Materials and Methods volunteers consumed all three probiotic preparations and a placebo control 志愿者随机服用三株益生菌制剂和安慰剂 biopsies were taken from the duodenum by standard flexible gastroduodenoscopy 采用标准的胃十二指肠胃镜方法对十二指肠进行 活组织检查 RNA extracted from these biopsies was hybridized to Affymetrix wholegenome expression arrays 从活组织提取RNA并进行Affymetrix（公司名称）全基因组表达分析杂交试验 Results Consumption of bacteria resulted in the differential expression of several hundred up to thousands of genes in vivo in human mucosa. 本试验中志愿者服用益生菌制剂后肠道黏膜上成百上千的基因表达发生改变。 2. Variation between persons was the largest source of transcriptome variation. 不同人服用同一益生菌制剂后黏膜组织上转录组的变化差异要大于同一人服用不同益生菌制剂所产生变化的差异大。 Fig.1 It is apparent that the differences between individuals are larger than the differences between interventions. 图1. 不同人服用同一益生菌制剂后黏膜组织上转录组的变化差异要大于同一人服用不同益生菌制剂所产生变化的差异大。 3. Mucosal responses to Lactobacillus acidophilus involve Regulation of Immune Response, Hormonal Regulation of Tissue Growth and Development, and Ion Homeostasis. 人肠道黏膜对嗜酸乳杆菌菌株的反应主要包括免疫反应调控、组织生长发育的激素调控和离子平衡。 4. Mucosal Responses to L. casei Involve Proliferation, Th1–Th2 Balance, and Hormonal Regulation of Blood Pressure. 人肠道黏膜对干酪乳杆菌菌株的反应主要包括细胞增殖、Th1–Th2细胞平衡和血压的激素调控。 5. Consumption of L. rhamnosus seemed to lead to differential expression of genes participating in signaling networks invol