急性低血压对前庭神经内侧核区谷氨酸和牛磺酸含量的影响.pdfVIP

生理学报 Acta Physiologica Sinica, April 25, 2006, 58 (2): 177-182 177 研究论文 急性低血压对前庭神经内侧核区谷氨酸和牛磺酸含量的影响 1 2 2 2 2 2,* 于海玲 ，安英 ，邴艳华 ，金清华 ，崔勋 ，金元哲 1 2 延边大学医学院 药理学教研室； 生理学教研室，延吉133000 摘 要：本实验用脑部微量透析法和高效液相色谱法观察急性失血诱发大鼠低血压时前庭神经内侧核(medial vestibular nucleus, MVN) 内氨基酸类神经递质谷氨酸(glutamate, Glu)和牛磺酸(taurine, Tau)含量的变化，以了解MVN 内这些氨基酸类 递质是否参与血压的调节。实验观察到，正常血压大鼠MVN 内Glu 和Tau 浓度在透析探针植入90 min 后趋于稳定，此时 Glu 的平均含量为(18.96±0.27) pmol/8 μl 透析液，Tau 的平均含量为(7.73±0.05) pmol/8 μl 透析液。动脉抽血1.5 ml 诱发急性 低血压时，同侧MVN 区Glu 含量增加(P 0.05) ，Tau 含量减少(P 0.05) 。用利多卡因(lidocaine)短时阻断前庭传入及用化 学方法永久破坏单侧前庭器官后，动脉抽血1.5 ml 使血压降低30% ，同侧MVN 内氨基酸类神经递质无明显改变。结果提 示，急性失血引起的低血压可通过前庭器官影响MVN 的活动，该区内氨基酸类递质可能参与这一活动的调节。 关键词：前庭内侧核；谷氨酸；牛磺酸；低血压 中图分类号：Q463; R338.2 Changes of glutamate and taurine released in the medial vestibular nucleus following acute hypotension 1 2 2 2 2 2,* YU Hai-ling , AN Ying , BING Yan-hua , JIN Qing-hua , CUI Xun , JIN Yuan-Zhe 1Department of Pharmacology; 2Department of Physiology, College of Medicine, Yanbian University, Yanji 133000, China Abstract: In order to understand whether some special amino acids in the medial vestibular nucleus (MVN) of rats are involved in the regulation of blood pressure, we used microdialysis technique and high performance liquid chromatography (HPLC) to measure the changes of glutamate and taurine in this central area. Acute hypotension was induced by hemorrhage from the femoral artery. It was observed that the basal release of glutamate and taurine in the MVN was stable about 90 min after the beginning of microdialysis. The basal release of glutamate was (18.96±0.27) pmol/sample (8 μl), and that of taurine was (7.73±0.05) pmol/sample (8 μl). Glutamate release increased (P 0.05) a