和Cyt位点的单链空间构型多样性研究黑脚硬.pdfVIP

动物学报 　50 (2) : 176 - 186 , 2004 A cta Zoologica S inica 　　　　　 　 Population structure of the blacklegged tick Ixodes scapularis re vealed by SSCP data using the mitochondrial Cyt b and the nuclear ITS1 markers Tonya R. M IXSON , Quentin Q. FAN G , D. Kelly McLAIN , James H. OL IV ER , J r Georgia Southern University , Department of Biolo gy and Institute of Arthropodolo gy and Parasitolo gy , PO Box 8042 , Statesboro , GA 304608042 , USA. Abstract 　The population genetic structure of the blacklegged tick Ixodes scapularis was analyzed using 853 individual ticks collected from 12 sites along the eastern seaboard of the United States. PCRbased DNA single strand conformation polymorphism ( SSCP) was used for analysis of one haploid locus , the mitochondrial cytochrome b ( Cyt b) , and one diploid locus , the internal transcribed spacer 1 ( ITS1) of nuclear ribosomal RNA. Seven haplotypes in the Cyt b locus and 13 genotypes in the ITS1 locus were identified. Population differentiation and isolation by distance were found. Dis tribution of haplotype and genotype frequencies across geographic regions suggests that two distinct populations exist along the Eastern Coast of the US. However , genetic variation among individuals within regions was large. This degree of vari ation suggests frequent gene flow between regions. Moreover , overall genetic diversity among individuals was much higher in ticks from the southern population [ Acta Zoologica Sinica 50 (2) : 176 - 186 , 2004 ]. Key words 　Blacklegged tick , Population genetics , Genetic variation , Gene flow , SSCP 利用 ITS1 和 Cyt b 位点的 DNA 单链空间构型多样性研究黑脚硬