微波辅助萃取-GC-MS联用快速分析.docVIP

微波辅助萃取-GC-MS联用快速分析蔬菜样品中痕量二嗪农 苏颖仪 邹兰 李敏健 培刚 谭宝莹 胡丹维 鲍雅丹 张卓恒 林智海 朱艳 （化学与化学工程学院，99级，广州510275） 指导老师：李攻科 教授 摘要：随着化肥、农药、塑料农膜等农用化学品施用量的增加，给生态环境造成严重的污染和破坏。有机磷农药由于所含的不稳定和水溶性化合物较多，它们极易被植物根部吸收，而又能以多种化学或光化学的途径较快地降解，在生物链中的积累较低，因此，被广泛应用于农作物病虫害防治领域。与此同时，有机磷农药对生物机体机能的毒害作用更是不容忽视的问题。建立有效、快速、灵敏的有机磷农药痕量残留的检测技术成为当前日益关注的课题。 有机磷农药必须先经过一定的预分离才能进行分离检测，传统的预分离手段有索氏提取、超声提取、机械振荡提取等，但是这些方法耗时，步骤繁琐，溶剂用量大。微波辅助萃取（Microwave-Assisted Extraction，MAE）是利用微波能来强化萃取过程、提高萃取效率的一种新技术。它具有高效节能，节省溶剂，轻污染，多份试样同时处理的特点，因此现在倍受分析工作者的青睐。 本文建立了微波辅助萃取-GC-MS联用技术分析蔬菜样品中二嗪农的分析方法。选取合适的萃取溶剂类型，萃取溶剂体积和微波辅助萃取优化时间。用优化的实验条件测定了200 ng/g合成蔬菜样品。本方法省时省溶剂，操作简便，适合于环境样品中有机磷农药的快速测定。 关键词：微波辅助萃取（MAE），气相色谱-质谱联用（GC-MS），有机磷，二嗪农，蔬菜。 Determination of Diazinon in Vegetable by GC-MS Using Microwave-Assisted Extraction Su Yinyi Zou Lan Li Minjian Pei Gang Tan Baoying Hu Danwei Bao Yadan Zhang Zhuoheng Lin Zhihai Zhu Yan (School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China) Supervisor: Prof. Li Gongke Abstract: With the increasing use of fertilizers, pesticides and plastic membrane, environment is polluted and damaged seriously. Organophophorus pesticides which contain unstable and water-soluble compounds are easily absorbed by roots. As a result of their relatively rapid degradation and low accumulation in the biological food chain, organophosphorus pesticides are widely applied to a variety of crops, including green vegetables and fruits. Hence, the monitoring of pesticide residues in agricultural products and in environmental matrices has become a priority field in pesticide research and analysis. Traditional pretreatment procedures such as Soxhlet extraction, ultrasonic extraction and direct extraction with organic solvent or hot water are time-consuming and produce large amount of solvent waste, as very efficient in extracting the target analyte. Microwave-assisted Extraction (MAE) is a new extraction technique, which can improve the extraction efficiency by microwave energy and keep the