低温处理对大蒜茎尖离体培养芽形成及生长的影响.pdfVIP

中国农业大学学报 　2003 ,8 (4) :24～26 Journal of China Agricultural University 低温处理对大蒜茎尖离体培养芽形成及生长的影响 1 2 曲英华 　高树英明 ( 1 中国农业大学 水利与土木工程学院 ,北京 100083 ; 2 日本山形大学 农学部, 鹤岗 997) 摘 　要 　为克服大蒜茎尖离体培养增殖率低的问题, 对准备切取茎尖组织进行离体培养的大蒜种球以及切取的带 ( ) 有 1 , 2 片叶原基的大蒜茎尖组织 外植体 进行了低温处理, 结果表明, 低温处理促进了大蒜茎尖离体培养时芽的 形成及生长。对外植体的低温处理比对种球的低温处理效果好, 60 d 低温处理后, 外植体低温处理区的新芽形成 数是种球低温处理区的 10 倍, 平均每个茎尖形成新芽 22 2 个; 种球低温处理和外植体低温处理并用时, 没有产生 累加效应, 相反, 外植体低温处理的效果, 被种球低温处理削弱。 关键词 　大蒜; 离体培养; 外植体; 低温处理 中图分类号　S 6334 　　　　文章编号 (2003) 04 0024 03 　　　　文献标识码 　A Effects of low temperature on shoot proliferation and shoot grow in garlic shoottip culture 1 2 Qu Yinghua , 　Takagi Hiteaki ( 1Water Conservancy and Civil Engineering College , China Agricultural University , Beijin g 100083 , China ; 2 J apan Yama gata University , Turuoka 997 , J apan) Abstract 　In order to overcome the problem of the low fre quency of proliferation of garlic shoottips culture , the garlic bulbs and the shoottips ( explants ) with 1 , 2 leaf primordiums need to be treated under low temp erature . The forming and growing of the shoot of garlic shoottip cultures were promoted under low temp erature . The effect of the treatment on explants was better than that of on bulb. During the s ame p eriod of 60 days under low temp erature , the number of shoots was 10 times as much as that of bulbs . In avera ge , each shoot form 22 2 new shoots . Accumulative effects were not obs e