高脂饮食肥胖大鼠胰岛细胞胰岛素抵抗机理的探析.pdfVIP

2005 7 20 85 27 Natl M ed J C h ina, Ju ly 20, 2005, V o l 85, No127 # 1907# # # 邬云红 李秀钧 李宏亮 王煜 张玄娥 柯柳 张祥迅 ( IR), , 30 W ista , 15, 20 , ; ; ( IR c) 12( IRS-1IR S-2) ( 1) ( IS I) , ( 362 pg/m l? 58 pg/m l vs 291 pg/m l? 35 pg/m l, P 0105; 442 pg /m l? 56 pg/m l vs 287 pg/m l? 48 pg /m l, P 01 05) ( 2) ( G SIS), 161 7 mm ol/L A, ( 3 ) IR c IR S-1 IR S-2 IR cIRS-2 28% 22% ( P 01 01) , IR IR, ; ; A study on the m echan ism of islet cell in sulin resistance in h igh- fat- diet obese rats W U Yun-hong, LI X iu-jun, LI H ong-liang, WANG Yu, ZH AN G X uan-e, KE L iu, ZHANG X iang-x un1 Insulin and D iabe es R esearch Uni, D ep ar m en of E ndocrinology, W es Ch ina H o sp i a,l S ich uan Univ ersi y, Chengdu 610041, Ch ina Corresp onding au hor: LI X iu-jun, Ema il: xj l629@ yahoo 1com 1cn A bstract O bj ective T o study the changes of insulin signa l t ansduction in isle t ce lls of high-fat- d ie t ats w ith pe iphe al insulin esistance ( IR) 1 M eth ods T h i ty m ale W ista ats we e andom ly d iv ided into 2 equal g oups: h igh-fa t-diet g oup and cont o l g oup to be fed w ith h igh-fa t food and no m al food espectively1 Twenty w eeks afte the ats w e e sac ificed1 T he conten ts of insulin and g lucagon in hom og enate of panc eas w e e detected du ing isle t ce ll pe ifusion, and insu lin ecepto ( IR c) and insu lin ecepto subst a tes ( IR S-1 and IRS-2) we e detected by mi m unoh istochem ist y1 R esu lts ( 1) T he insulin sensitive index ( IS I) was significantly dec eased in the h igh-fat-d ie t a ts in compa ison w ith the no ma l a ts, wh ile the contents of g lucago