水分胁迫下夏玉米根叶保护酶活性变化和其对膜脂过氧化作用的影响.pdfVIP

中国农业科学 2005,38(5):922-928 Scientia Agricultura Sinica 水分胁迫下夏玉米根叶保护酶活性变化及其对 膜脂过氧化作用的影响 1 1 2 1 2 葛体达 ，隋方功 ，白莉萍 ，吕银燕 ，周广胜 1 2 （莱阳农学院农学系，莱阳 265200 ； 中国科学院植物研究所植被数量生态学开放研究实验室，北京 100093 ） 摘要：利用防雨旱棚，研究了水分胁迫下夏玉米根叶保护酶系活性变化及其对膜脂过氧化作用的影响。结果 表明：在水分胁迫下，玉米根系、叶片保护酶系SOD、CAT、POD活性均在生长发育前中期显著升高而后期下降， 根系SOD、CAT和POD活性小于叶片。膜脂过氧化产物MDA含量随水分胁迫程度加剧而增加，且根系MDA含量小于 叶片MDA含量。研究还表明，在水分胁迫下，玉米根系保护酶活性及膜脂过氧化作用对水分胁迫的反应和叶片保 护酶活性及膜脂过氧化作用对水分胁迫的反应呈正相关关系，且绝大多数的相关系数达显著或极显著水平。此外， 叶片与根系中可溶性蛋白质含量降低。水分胁迫导致夏玉米果穗性状恶化，经济产量大幅下降。造成减产的主要 原因是每穗粒数的减少和百粒重的下降。 关键词：玉米；水分胁迫；保护酶活性；膜脂过氧化 Effects of Water Stress on the Protective Enzyme Activities and Lipid Peroxidation in Roots and Leaves of Summer Maize 1 1 2 1 2 GE Ti-da , SUI Fang-gong , BAI Li-ping , LÜ Yin-yan , ZHOU Guang-sheng 1 2 ( Department of Agronomy, Laiyang Agricultural College, Laiyang 265200; Laboratory of Quantitative Vegetation Ecology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093 ) Abstract: Using water proof installations, a systematic study was conducted determine the effects of water stress on the protective enzyme activities and lipid peroxidation were determined. The results showed that: Under water stress, the activities of superoxide dismutase(SOD), catalase(CTA),and peoxidase(POD) in leaves and roots increased sharply at prophase and metaphase growth stages such as male tetrad stage, while declined at the evening developmental stages such as milky stage.T