抗原致敏的DC联合CIK对肝癌细胞杀伤活性的研究-一、.docVIP

一、一审综合评价： 1﹒创造性 国际首报[ ] 国内首报[ ] 有创新性[] 一般[√] 重复性工作[ ] 2﹒科学性 设计严谨 是[] 否[√] 资料齐全 是[] 否[√] 研究方法得当 是[√] 否[ ] 结果可靠 是[√] 否[ ] 统计学处理 合理[√] 否[ ] 结论合理 是[√] 否[ ] 3﹒实用性 有重大应用和推广价值[ ] 有指导和参考价值[√] 一般[] 4﹒可读性 文题贴切 是[√] 否[ ] 层次清楚 是[√] 否[ ] 逻辑准确 是[√] 否[ ] 论点明确 是[√] 否[ ] 文笔通顺 是[ ] 否[√] 图表设计合理是[√] 否[ ]英文摘要通畅达意 是[] 否[√] 5﹒总体评价 优秀（国际先进水平）[ ] 良好（国内先进水平）[ ] 较好[] 一般[√] 6﹒处理意见 优先发表[ ] 按常规发表[ ] 修改后再审[√] 修改后发表[ ] 退稿[ ] 是否需要配发述评或编者按 是[ ] 否[ ] 7﹒刊用形式 述评[ ] 专家笔谈[ ] 论著[√] 循证医学[ ] 短篇论著[ ] 临床经验[ ] 病例报告[ ] 临床病例（病理）分析[ ] 综述[ ] 讲座[ ] 学术动态[ ] 新技术 新方法[ ] 二、对文稿的评审意见： ⑴.本文观察了肝癌细胞抗原致敏的树突状细胞(DC)与细胞因子诱导的杀伤细胞(CIK)共培养后的细胞增殖活性、表型的变化及其对肝癌细胞HepG2的杀伤活性，结果发现：肝癌细胞抗原致敏的DC与CIK细胞共培养后，Ag-DC-CIK细胞群增殖活性明显增强，且高表达CD3+CD8+、CD3+CD56+双阳性细胞，其增殖活性和杀伤活性较单纯的CIK细胞更高(P0.05)。实验设计合理，内容充实。 ⑵.中英文摘要中结论部分不一致，建议作相应修改。 ⑶.中英文关键词中：杀伤作用与liver cancer cells不一致，建议作相应修改。 ⑷.朗读 显示对应的拉丁字符的拼音 Study of killing activity of antigen-pulsed DC combined CIK against hepatocarcinoma cells [Abstract] Objective:To observe the proliferation activities ,phenotype changes and its killing activity against HepG2 hepatocarcinoma cells of the liver cell antigen-pulsed dendritic cells (DC) cultured with cytokine-induced killer cells (CIK). Method:Peripheral blood mononuclear cells (PBMNC) were isolated from healthy donors, then DC and CIK were induced.The Ag-DC were co-cultured with CIK (Ag-DC-CIK)and the phenotype and proliferation the CIK and the Ag-DC-CIK cells were observed.Their cytotoxicity activity against HepG2 hepatocarcinoma cells were detected by MTTassay.Results:The proliferation of Ag-DC-CIK cells group was significantly enhanced and express CD3+CD8+、CD3+CD56+ double positive cells,and their proliferation and cytotoxic activity were higher than the CIK cells (P 0.05). ConclusionTumor antigen-pulsed DC were cultured with CIK cells are highly effective immune cells. [Keywords] Dendritic cells; cytokine induced killer cells; liver cancer cells 原发性肝癌是我国常见的恶性肿瘤之一，大多数患者确诊时已