脑源性神经营养因子对小鼠回肠及结肠平滑肌收缩活动的影响.docVIP

脑源性神经营养因子对小鼠回肠及结肠平滑肌收缩活动的影响及其机制 陈飞雪，于岩波，王鹏，左秀丽，李延青 (山东大学齐鲁医院消化内科，济南 250012) 摘要：目的 观察不同浓度脑源性神经营养因子(BDNF)对小鼠离体回肠和结肠平滑肌肌条收缩活动的影响，探讨蛋白酪氨酸TrkB)-磷脂酶C(PLC)/三磷酸肌醇（IP3）信号通路在BDNF影响肠道平滑肌收缩过程中的作用。方法 制备小鼠回肠、近端结肠和远端结肠平滑肌纵肌条观察不同浓度BDNF (1×10-81×10-7 mol/L)对小鼠离体肠道肌条收缩活性的影响，并分别观察TrkB抗体、新霉素及肝素种TrkB-PLC/IP3信号通路阻滞剂孵育肌条后，BDNF对肌条收缩活动的影响。结果 BDNF (1×10-81×10-7 mol/L)对小鼠回肠和结肠平滑肌离体肌条的收缩具有明显的兴奋效应，(P0.05)。TrkB抗体新霉素及肝素明显BDNF (1×10-7 mol/L)对肌条收缩的兴奋作用，其张力效应值与对照组相比均有统计学意义(P0.05)。结论 BDNF兴奋小鼠肠道平滑肌肌条的收缩活动TrkB-PLC/IP3 信号通路在BDNF影响肠动力过程中发挥重要作用关键词脑源性神经营养因子；信号通路；平滑肌；收缩；小鼠中图分类号: R574 文献标码: ABrain-derived neurotrophic factor influences contractile activity in the isolated ileum and colon of mice CHEN Fei-xue, YU Yan-bo, WANG Peng, ZUO Xiu-li, LI Yan-qing （Department of Gastroenterology, Qilu Hospital of Shandong University, Jinan 250012, China） Abstract: Objective To elucidate the direct effects of brain-derived neurotrophic factor (BDNF) on contraction of mice intestinal muscle strips and to investigate whether the effect of BDNF on intestinal contraction is mediated through the tyrosine kinase receptor B (TrkB)- phospholipase C (PLC)/ inositol triphosphate (IP3) pathway. Methods Longitudinal muscle (LM) strips of ileum, proximal colon and distal colon from C57bl/6 mice were prepared. The contraction of LM was recorded with tension transducer. To investigate the contractile effects of BDNF on LM strips, two different doses of BDNF (1×10-8 and 1×10-7 mol/L) were added to the muscle strips. To investigate the effects of antagonists of TrkB-PLC/IP3 signal pathway on the contraction of muscle strips induced by BDNF, LM strips were pretreated with TrkB (1×10-8 mol/L), neomycin (1×10-5 mol/L) and heparin (1×10-5 mol/L) before administration of BDNF. Results BDNF excited the contraction of LM from mice ileum, proximal colon and distal colon (P0.05) of mice. Pretreatment with TrkB (1×10-8 mol/L), neomycin (1×10-5 mol/L) and heparin (1×10-5 mol/L) dramatically attenuated the excitatory effects of BDNF (10-7 mol/